LOCUS       sequence-239680-       10910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the H031 HIV-1 isolate; 
            QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-H031-QES.i07.c09
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(419..437)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        505..609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(610..678,679..1470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(828..847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1641..2229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2130..2149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2660..2805
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2848..3885
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3883..3902)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3927..3975
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4629..5008
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5009..5212
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5163..5183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5209..5233
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7202..7246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7533..7667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7557..7576)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7611..7630
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7810..7832)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8392..10181
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc
       61 ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc
      121 tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga
      181 aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc
      241 ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc
      301 ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg
      361 tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
      421 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      481 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      541 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      601 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      661 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      721 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      781 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      841 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      901 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      961 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1021 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1081 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1141 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1201 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1261 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1321 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1381 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1441 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1501 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1561 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1621 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1681 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1741 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1801 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1861 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1921 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1981 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2041 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2101 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2161 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2221 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga
     2281 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     2341 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     2401 aacgccagca agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg
     2461 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
     2521 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccctg
     2581 cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt
     2641 gcatgtcttt agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc
     2701 gaacacgcag atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac
     2761 gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt
     2821 gccgcagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc
     2881 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     2941 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     3001 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     3061 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     3121 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     3181 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     3241 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     3301 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     3361 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     3421 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     3481 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     3541 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     3601 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     3661 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     3721 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     3781 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     3841 cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac
     3901 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
     3961 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4021 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     4081 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     4141 ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg
     4201 ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc
     4261 atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg
     4321 tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca
     4381 agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt
     4441 gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg
     4501 gcaattagcc atattagtca ttggttatat agcataaatc aatattggct attggccatt
     4561 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc
     4621 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     4681 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     4741 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4801 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4861 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
     4921 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
     4981 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
     5041 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     5101 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
     5161 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     5221 gaaccgtcag atctctagaa gctgggtacc agctgctagc gccaccatgc cgatgggatc
     5281 acttcaacct ttggcaaccc tgtatctcct ggggatgttg gttgccagcg tcctcgcagc
     5341 agaggacctc tgggttacag tttattatgg agtgccagtc tggaaggacg cagacactac
     5401 cctcttctgc gcttcagagg ctaaagcgta tgatactgag gcacacaacg tctgggccac
     5461 ccacgcttgc gtaccgacgg acccaaaccc gcaggagata gtcctgcaaa acgtgaccga
     5521 gggctttaat atgtggaaaa acaatatggt agaacagatg cacgaagaca tcatctcttt
     5581 gtgggatcaa agcctcaagc cgtgtgttaa gcttacccca ctttgcgtta cactgaattg
     5641 cactactgcc aatgcgaaca gtacagctaa cgcaacctca acaacgtcta ccacgtctac
     5701 agatatgaag aactgttcat ttaacattac aactgacatg agggacaaag ttcagaagca
     5761 acacgcattg ttttattcac tcgacctggt ccctatagat aattccaata atagttatag
     5821 acttataagt tgtaacacta gtgtcatcac ccaagcctgt ccaaaaatca gctttgagcc
     5881 catccctatc cattactgcg ctccggcggg atacgcaatt atcaagtgta atgataagaa
     5941 ctttgccggc ataggaacct gcaagaatgt gagtacggtc cagtgtactc acggcgtaag
     6001 gcccaccatg agtacccagc ttctcttgaa cggtggactc accgagggcg aggtcgtcat
     6061 caaatctgcc aatttcagcg acaacacaaa gacaattata gtacagttgt ctgagtcaat
     6121 cgagataaac tgcacacggc caagcaacaa tacacgaaaa tcaattacta tagggccagg
     6181 tcgagcattc tacacgaccg gggacatcat aggggacata aggcgcgccc attgtaatat
     6241 atctggcgcg aaatggaaca acaccctccg gcgcatcgca gaaaagttga aagtccaata
     6301 cgaaaacaga acaatcgtgt tcaacgcctc ttcaggcggt gatcctgaag ttgtcatgca
     6361 cacctttaat tgtggtgggg aatggttcta ctgcaatacc actcaactgt tcaattcaac
     6421 cttcaatagt acctgggaaa gagaaacaaa tacgacagaa ctcaatacaa acatcacaat
     6481 tacactcccc tgcagaatca aacagataat caatatgtgg caagaggtcg ggaaagccat
     6541 gtacgcgcct cctatccgag gccaaataag gtgtagtagc aacataacgg ggcttcttct
     6601 cacccgagat ggcagccata ataataacac aacggagaca ttcagaccag gtggtggtga
     6661 catgagagat aattggcgca gtgagctgta caaatataag gttgttaaaa tagagcctct
     6721 cggtatcgca cctactaagg caaagaggcg agtagtacaa cgggagaaaa gggctgtagg
     6781 acttggggcg gtgtttctgg gatttctcgg ggcggcagga tctacaatgg gggctgccag
     6841 catcacgctg accgtgcagg cgcggcagct gctgtcaggg atcgttcagc agcagcgcaa
     6901 ccttcttaat gcaatcgagg ctcagcagca ccttctgcag ttgacagtat gggggataaa
     6961 acagcttcag gctcgggtgc tggctataga gcggtacttg aaagaccagc agctccttgg
     7021 tatatgggga tgcagcggca agcttatatg tacgacggct gtgccctgga acgcctcctg
     7081 gtccaacaaa aaccttagtc agatttggga caatatgacg tggatggagt gggagaagga
     7141 gatagacaat tacaccgggt tgatttacaa ccttctggag atctcacaaa atcagcagga
     7201 gaaaaatgag caagagcttc ttgagctcga caagtgggcg agtctctgga attggtttga
     7261 catcacgagc tggctctggt atatcaagat cttcatcatc ataataggtg gccttatcag
     7321 cttgagaatc atctgcgcag tcatcaacat agtgaataag gtgagacaac gctactctcc
     7381 actgagtttc cagacgctgc ttccgacccc acgagggccg gataggccgg aacgaatcga
     7441 agaggaggat cgggaccggg atagagatac cagcgtaagg ttggtaacgg gcttcctcgc
     7501 gcttatttag taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
     7561 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7621 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     7681 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     7741 acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga
     7801 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     7861 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     7921 agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     7981 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     8041 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     8101 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     8161 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     8221 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     8281 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     8341 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     8401 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     8461 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     8521 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     8581 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     8641 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     8701 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     8761 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     8821 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     8881 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     8941 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     9001 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     9061 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     9121 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     9181 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     9241 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     9301 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     9361 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     9421 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     9481 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     9541 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     9601 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     9661 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     9721 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     9781 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     9841 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     9901 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     9961 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    10021 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
    10081 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
    10141 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
    10201 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
    10261 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
    10321 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
    10381 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
    10441 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
    10501 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
    10561 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
    10621 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
    10681 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
    10741 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
    10801 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
    10861 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
//
