LOCUS       sequence-239682-       10274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH115 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH115gp160
FEATURES             Location/Qualifiers
     source          1..10274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(716..734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        802..906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(907..975,976..1767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1125..1144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1938..2526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2427..2446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2957..3102
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3145..4182
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4180..4199)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4224..4272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4926..5305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5306..5509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5460..5480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5506..5530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8184..8318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8208..8227)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8262..8281
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8461..8483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact tggacgtttt
       61 tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc cggggctcct
      121 ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt atcaccccct
      181 cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc cttctctcct
      241 aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat
      301 caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc
      361 tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga
      421 aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa
      481 ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc
      541 gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc
      601 aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga
      661 ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
      721 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      781 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      841 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      901 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      961 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1021 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1081 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1141 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1201 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1261 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1321 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1381 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1441 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1501 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1561 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1621 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1681 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1741 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1801 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1861 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1921 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1981 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2041 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2101 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2161 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2221 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2281 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2341 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2401 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2461 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2521 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc
     2581 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     2641 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     2701 gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga
     2761 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     2821 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt
     2881 catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca
     2941 tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     3001 cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg
     3061 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
     3121 gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     3181 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     3241 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     3301 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     3361 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     3421 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     3481 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     3541 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     3601 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     3661 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     3721 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     3781 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     3841 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     3901 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     3961 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     4021 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     4081 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     4141 cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat
     4201 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     4261 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4321 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     4381 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca
     4441 ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt
     4501 tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg
     4561 gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct
     4621 gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc
     4681 tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     4741 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
     4801 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
     4861 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
     4921 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4981 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5041 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5101 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5161 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
     5221 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
     5281 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
     5341 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     5401 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     5461 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     5521 ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgccga tgggctccct
     5581 tcaaccgctc gctacgctgt atcttctcgg catgttggtc gctagtgtgc tggctgtcgg
     5641 taatctttgg gttacagtgt actatggtgt accagtgtgg agagaggcaa ccactactct
     5701 tttctgtgct tccgacgcca aagcttatga cgcggaagtt cacaatgttt gggcaacgca
     5761 tgcctgcgta cctacagacc ccaaccccct cgaaattgat ctcaaaaatg ttactgagaa
     5821 ctttaatatg tgggaaaata agatggtgga tcaaatgcat gaagatgtaa tcagtctttg
     5881 ggaccagagc ttgaagccat gcgtgaaact gacgcccttg tgtgtgacat tggaatgctc
     5941 taatgtaacc ttggaacggt caaatgtttc aagtagtaat gacacccaga atagaacgca
     6001 ccatgagtcc atcaaagaga tgaaaaattg cagcttcaac gctaccaccg agctccgaga
     6061 taaaaagcaa aaggtgcacg cactctttta tcgcttggac atcgtaccgt tggacgagaa
     6121 gaaaaatagc agcgaaaatt cttccgaaag ttacaggctc atcaattgta ataccagtgc
     6181 aataacacag gcgtgtccaa aggtcacttt cgatccgatc ccaatccact actgcactcc
     6241 agcaggatat gcgatactca agtgtaagga caagatgttc aatggaaccg gaccttgcca
     6301 taatgtgtca acagtccagt gtacgcacgg aattaagcca gtggtatcta cgcagcttct
     6361 cttgaacggt agcctcgccg agggggagat aataattcga tcagagaact tgacagacaa
     6421 tgcaaagata atcatagtac atctgaatca atctgtagaa atcaactgca cacggccctc
     6481 caataacact agacagagta tcagaatcgg gccggggcaa accttttacg ccactggaga
     6541 gatcatcggc gacattagac aggcgcactg taacatcagc agggcgaggt ggaatgaaac
     6601 ccttcaaaag gtgggtaaaa aactcgctga gcactttcag aataagacaa tcaagtttaa
     6661 ctcatcttca ggaggagatc tcgaaattac aactcacagt ttcaattgca gaggcgagtt
     6721 tttctattgc aacacttcag gtctcttcaa cggcacatac atgcccaacg gcacttttat
     6781 cggggccgaa ggcaattcaa ctataacaat tccttgccga ataaagcaaa tcattaatat
     6841 gtggcaagaa gtaggcagag ccatgtacgc ccctccaatc gctggcaata taacctgtaa
     6901 tagtaatatc actggcctgc tcctcgtcag ggacgggggt aatatcattg ggcaaaataa
     6961 tactgagacg ttcaggccgc ttgggggtaa catgaaagat aattggagaa gtgaacttta
     7021 taaatataag gtagtggaaa taaagccctt gggcatagct cccaccgacg cgaagcggag
     7081 ggtcgtagaa cgagagaagc gcgcggtggg tttgggtgcc gtatttctgg gattccttgg
     7141 agccgctgga tccacaatgg gggcggcgtc tctcacgctc acagttcaag cgaggcaact
     7201 tctgagtgga atcgtacagc aacagaacaa cctgcttaga gcgatcgaag ctcagcagca
     7261 tttgcttcaa ttgactgtat gggggatcaa gcagctgcag gccagggtgc ttgccattga
     7321 gcggtacttg caagaccagc aattgctggg gatctggggt tgtagcggaa aacttatttg
     7381 cacgaccgcc gtaccgtgga acgattcatg gagcaacaag tccttgacag acatctggaa
     7441 cggcacgaca tggatgcaat gggataagga gattagcaca tacaccgaca tcatatattc
     7501 cctgcttgaa gactcacaga ttcaacagga aaagaatgag aaggacctgt tggctctgga
     7561 ctcatggaaa aatttgtgga attggttctc catcacaaac tggctgtggt atattaagat
     7621 attcataatg attgtagggg ggcttatcgg cttgcgcatt atttttgctg tgctctccat
     7681 agtgaatcgg gtaaggcaag gatactcccc gctctccttc cagacactta tacctaatag
     7741 cggcggtcct gaccggttgg gtcgcattga agaagaaggc ggcgaacagg acaaagacag
     7801 atccatccgc ctcgtatctg gattcctcgc attggcgtgg gacgatctcc ggtccctctg
     7861 tctgtttagt tatcaccgcc tgagagattt cattctcctg actgcacgag tggtggagct
     7921 gctgggacgg aatagccttc gagggctcca gcgaggttgg gaggccctta agtatctggg
     7981 ctccctggta cagtactggg ggcaagaact taagaagagc gccatttcac tgttcgatac
     8041 catagctatt gccgtggcag aaggtactga tcggatcata gagcttgtac aaggcttttg
     8101 tagggcgatc tataacattc cgagacgcat tcgccaggga tttgaggcgg cattgcagta
     8161 gtaactcgag gccggcaagg ccggatccag acatgataag atacattgat gagtttggac
     8221 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     8281 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     8341 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     8401 aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     8461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     8521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag
     8581 gtcgactcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg
     8641 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     8701 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     8761 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     8821 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     8881 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     8941 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     9001 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     9061 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     9121 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     9181 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     9241 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     9301 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     9361 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     9421 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     9481 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     9541 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     9601 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     9661 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     9721 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     9781 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     9841 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     9901 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     9961 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
    10021 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
    10081 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
    10141 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
    10201 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10261 gtatatgcta tcct
//
