LOCUS       sequence-239691-       11663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the H031 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-H031gp160
FEATURES             Location/Qualifiers
     source          1..11663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(896..964,965..1756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1114..1133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1927..2515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2946..3091
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3134..4171
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4169..4188)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4213..4261
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4915..5294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5295..5498
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5449..5469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5495..5519
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7488..7532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7819..7953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7843..7862)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7897..7916
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8096..8118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8678..10467
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg
       61 gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc
      121 tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg
      181 tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc
      241 aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc
      301 tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc
      361 ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat
      421 cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa
      481 catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt
      541 tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag
      601 acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta
      661 tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg
     2581 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     2641 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     2701 gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     2761 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     2821 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg
     2881 cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt
     2941 ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc
     3001 agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     3061 acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg
     3121 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     3181 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     3241 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     3301 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     3361 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     3421 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     3481 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     3541 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     3601 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     3661 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     3721 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     3781 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     3841 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     3901 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     3961 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     4021 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     4081 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     4141 tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4201 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     4261 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4321 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4381 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg
     4441 ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg
     4501 gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg
     4561 gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa
     4621 acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa
     4681 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     4741 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     4801 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     4861 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt
     4921 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5161 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5221 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
     5281 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
     5341 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5401 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
     5461 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct
     5521 ctagaagctg ggtaccagct gctagcgcca ccatgccgat gggatcactt caacctttgg
     5581 caaccctgta tctcctgggg atgttggttg ccagcgtcct cgcagcagag gacctctggg
     5641 ttacagttta ttatggagtg ccagtctgga aggacgcatc cactaccctc ttctgcgctt
     5701 cagaggctaa agcgtatgat actgaggcac acaacgtctg ggccacccac gcttgcgtac
     5761 cgacggaccc aaacccgcag gagatagtcc tgcaaaacgt gaccgagggc tttaatatgt
     5821 ggaaaaacaa tatggtagaa cagatgcacg aagacatcat ctctttgtgg gatcaaagcc
     5881 tcaagccgtg tgttaagctt accccacttt gcgttacact gaattgcact actgccaatg
     5941 cgaacagtac agctaacgca acctcaacaa cgtctaccac gtctacagat atgaagaact
     6001 gttcatttaa cattacaact gacatgaggg acaaagttca gaagcaacac gcattgtttt
     6061 attcactcga cgtggtccct atagataatt ccaataatag ttatagactt ataagttgta
     6121 acactagtgt catcacccaa gcctgtccaa aaatcagctt tgagcccatc cctatccatt
     6181 actgcgctcc ggcgggattc gcaattatca agtgtaatga taagaacttt gccggcatag
     6241 gaacctgcaa gaatgtgagt acggtccagt gtactcacgg cgtaaggccc gtggtcagta
     6301 cccagcttct cttgaacggt ggactcaccg agggcgaggt cgtcatcaaa tctgccaatt
     6361 tcagcgacaa cacaaagaca attatagtac agttgtctga gtcaatcgag ataaactgca
     6421 cacggccaag caacaataca cgaaaatcaa ttactatagg gccaggtcga gcattctaca
     6481 cgaccgggga catcataggg gacataaggc gcgcccattg taatatatct ggcgcgaaat
     6541 ggaacaacac cctccggcgc atcgcagaaa agttgaaagt ccaatacgaa aacagaacaa
     6601 tcgtgttcaa cgcctcttca ggcggtgatc ctgaagttgt catgcacacc tttaattgtg
     6661 gtggggaatt tttctactgc aataccactc aactgttcaa ttcaaccttc aatagtacct
     6721 gggaaagaga aacaaatacg acagaactca atacaaacat cacaattaca ctcccctgca
     6781 gaatcaaaca gataatcaat atgtggcaag aggtcgggaa agccatgtac gcgcctccta
     6841 tccgaggcca aataaggtgt agtagcaaca taacggggct tcttctcacc cgagatggca
     6901 gccataataa taacacaacg gagacattca gaccaggtgg tggtgacatg agagataatt
     6961 ggcgcagtga gctgtacaaa tataaggttg ttaaaataga gcctctcggt atcgcaccta
     7021 ctaaggcaaa gaggcgagta gtacaacggg agaaaagggc tgtaggactt ggggcggtgt
     7081 ttctgggatt tctcggggcg gcaggatcta caatgggggc tgccagcatc acgctgaccg
     7141 tgcaggcgcg gcagctgctg tcagggatcg ttcagcagca gcgcaacctt cttaatgcaa
     7201 tcgaggctca gcagcacctt ctgcagttga cagtatgggg gataaaacag cttcaggctc
     7261 gggtgctggc tatagagcgg tacttgaaag accagcagct ccttggtata tggggatgca
     7321 gcggcaagct tatatgtacg acggctgtgc cctggaacgc ctcctggtcc aacaaaaacc
     7381 ttagtcagat ttgggacaat atgacgtgga tggagtggga gaaggagata gacaattaca
     7441 ccgggttgat ttacaacctt ctggagatct cacaaaatca gcaggagaaa aatgagcaag
     7501 agcttcttga gctcgacaag tgggcgagtc tctggaattg gtttgacatc acgagctggc
     7561 tctggtatat caagatcttc atcatcataa taggtggcct tatcagcttg agaatcatct
     7621 gcgcagtcat caacatagtg aataaggtga gacaacgcta ctctccactg agtttccaga
     7681 cgctgcttcc gaccccacga gggccggata ggccggaacg aatcgaagag gaggatcggg
     7741 accgggatag agataccagc gtaaggttgg taacgggctt cctcgcgctt atttagtaac
     7801 tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     7861 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     7921 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     7981 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     8041 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     8101 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8161 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     8221 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     8281 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     8341 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     8401 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     8461 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     8521 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     8581 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     8641 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     8701 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     8761 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     8821 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     8881 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     8941 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     9001 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     9061 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     9121 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     9181 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     9241 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     9301 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     9361 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     9421 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     9481 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     9541 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     9601 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     9661 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     9721 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     9781 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     9841 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     9901 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     9961 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
    10021 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
    10081 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
    10141 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
    10201 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
    10261 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
    10321 atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat
    10381 acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt
    10441 gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg aatttaagga
    10501 ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc taatgttttc
    10561 caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt atgcccaatt
    10621 gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata caccaacagc
    10681 acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg cttttaatac
    10741 gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc tcatctccat
    10801 cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct tccaccatag
    10861 gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc accctgatat
    10921 tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc aaaacctcag
    10981 caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc cttagcgggc
    11041 caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat ggtgtaagac
    11101 gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag gtcttactac
    11161 ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac
    11221 tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg ttggaacctc
    11281 cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca tcaccctgac
    11341 cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc tctatcgctc
    11401 ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca aaataatcgg
    11461 cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc ggtggagacc
    11521 cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt ccacgacctc
    11581 tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta ccccggcggc
    11641 ctccactacc tcctcgaccc cgg
//
