LOCUS       sequence-239698-       11652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate 
            (containing SOSIP mutations); C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-gp160-754*
FEATURES             Location/Qualifiers
     source          1..11652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1175..2964
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3524..3546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3689..3823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3726..3745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3780..3799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6123..6147)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6144..6347)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6173..6193)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6348..6727
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7381..7429
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7454..7473
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7471..8508)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8551..8696)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(9127..9715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9207..9226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9886..10677,10678..10746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10509..10528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10747..10851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10919..10937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggccgccggg gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg
       61 agaggtcgtg gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc
      121 gggtctccac cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa
      181 gccgattatt ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg
      241 ggagcgatag agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg
      301 ggtcagggtg atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa
      361 ggaggttcca acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg
      421 agtcacgtag aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga
      481 ggtagtaaga cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt
      541 cgtcttacac cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct
      601 ggcccgctaa gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt
      661 gctgaggttt tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc
      721 aatatcaggg tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca
      781 cctatggtgg aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt
      841 gatggagatg agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat
      901 cgtattaaaa gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg
      961 tgctgttggt gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg
     1021 caattgggca tacccatgtt gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt
     1081 ggaaaacatt agcgacattt acctggtgag caatcagaca tgcgacggct ttagcctggc
     1141 ctccttaaat tcacctaaga atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt
     1201 cacgccccct tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg
     1261 tatcatatgc tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga
     1321 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     1381 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga
     1441 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga
     1501 tagcatatgc tacccagata tagattagga tagcatatgc tatccagata tttgggtagt
     1561 atatgctacc cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc
     1621 atatgctacc cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc
     1681 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc
     1741 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     1801 ctatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
     1861 gctacccatg gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa
     1921 caaccctgtg cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa
     1981 tcgcgcccct atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag
     2041 tggttttgtg ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta
     2101 ttacaccctt attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc
     2161 ccactccaca atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc
     2221 gtggagcccc gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg
     2281 gcgtccactc tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg
     2341 gtccctgcct gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt
     2401 gcccatagcc ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc
     2461 atggatttct attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc
     2521 caaggggttt gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt
     2581 ccaaatttta ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct
     2641 tactgttcac aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc
     2701 gaagattcag gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa
     2761 aatggttcac taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg
     2821 gggtgggaga tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg
     2881 cttcccgttg ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta
     2941 cccgggaagc atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct
     3001 aatgccctct tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt
     3061 gtagcctccc gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa
     3121 gagcttcagc caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa
     3181 gtctgctcca ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat
     3241 gtcaactaca gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca
     3301 gggcccagtt ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgcgaag
     3361 aaggggcaga gatgtcgtag tcaggtttag ttcgtccggg gcggggatcg atcctctaga
     3421 gtcgacctca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3481 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3541 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc cggatcataa tcagccatac
     3601 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3661 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3781 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccggcctt gccggcctcg
     3841 agttatcaca gaaacccgct aacaaggcgg gtacttctgc cccggtcctg ttcaccgtct
     3901 tcctcctcaa tccgctcggg tcggtccaat cccctggggt tgggggtatg ggtttgaaaa
     3961 gagagcggac tatacccctg cctaaccctg tggataacag acagcacagc aaacacaatc
     4021 ctcaaaccta tcaaaccccc gacaatcatt ataaatatct ttatatacca aagccaattt
     4081 gaaatgtcga accaattcca aagacttgcc cacttatcaa gggccagaag atcctgttca
     4141 ttcttctcct gctgattctg gctttcctcc aggagcccat agattatttg agtgtaattg
     4201 ctaatctcct tatcccactg caaccatgtc atattatccc agatctcgga gagatttcgg
     4261 ttagaccatg aggagttcca aggaacgtta gtgcaacaga tgagtttgcc tgaacaaccc
     4321 caaatcccca aaagttgttg gtccctaagg tatctttcca ccgcgagaac ccgcgcttgg
     4381 agttgcttta tgccccagac agtcaactta aggagatgtt gctgcgcctc aggagcccta
     4441 agaaggtttg actgttgttg cactataccg gacaggaggt tcctcgcctg tacggtcagg
     4501 gtcatactag ctgctcccat agtgctccca gccgccccca gaaaacccaa gaaaacggcc
     4561 ccaatgccga ccgcccgtcg ccttctcctc cttcccacta cccgtcgttt acatctcgtt
     4621 ggggcgacac ccaggggttc gattttaacc actttgtact tgtacaactc agaccgccag
     4681 ttgtctcgca tgtccccacc gccaggccgg aacgtctcag tggtagaatt agtgctgcct
     4741 ccatcgcgcg taagtataag gcccgttatg ttgcttacgc accgtattac tccctggata
     4801 ggcggggcgt acatcgcctg gccgattcgc tgccacatgt tgataatctg tttgatgcga
     4861 caaggaagtg ttatactatc gtttgatcct gtggagtttg atccctgaac actagtgtta
     4921 gatatccaag tggagttgaa caacccggat gtgttacaat aaaaaaactc cccgccgcag
     4981 ttaaatgaat gtgtagttac ttcaagatca cccccactac tgttcgcaaa tcggatgatg
     5041 gtattgttcc cgaaatgctt cctgagctgc ttgacaactt tacccagagt ctcattccat
     5101 gtggccttgc ttacattgca atgcgcctgt cggatgtcac ctattatatc tcccgtggca
     5161 tagaacgcct gacctggtcc gatgcgaatg gattttctgg tattattatt aggtcgggta
     5221 caattaatct ggacgggggt attaaattgg accaagatat tcttggcatt gttagtaata
     5281 ttctcactcc gtatcatgac ctcttcctct gcgaggctgc cattgaggag aagctgagtg
     5341 gataccactg gctttatgcc gtgggtacac tgaaccgtac tcacactggg acagggacca
     5401 gttccattaa acttcttatc cttgcatttc aagatcgcaa accctgccgg ggcgcaatag
     5461 tgtataggaa tcggctcaaa tgacaccttc gggcaggcct gggttattgc gctagtgtta
     5521 cagttgatca atctgtattc tttattggag ttattgctcc tgttcccctg gttttcgttt
     5581 atttgcacaa cgtcaagcct gtagaagagg ctgtacacct tctgcttttt atctcgcaac
     5641 tccgttgtca tattaaagct gcaattcttc agttcgccgc gcatgtcgtc cgtaatgtta
     5701 ttcgttacgt tagtacattg cagagttacg cagaggggcg taagtttaac gcaaggttta
     5761 aggctctggt cccaaagact gataatatcc gtatgcattt gttctaccat gttattcttc
     5821 cacatgttga attcctctgt tacattctca aggtgaatct cctgaggatt tgggtctgtc
     5881 gggacgcagg catgcgttgc ccaaacattg tgcttttccg tttcgtatgc cttcgcgtcg
     5941 gatgcgcaaa aaagcgttgt ttctgcgtct ttccaaacgg gtactccata gtagacagtt
     6001 acccacaagt tttctgcggc aagcacagat gcgaccaaca tcccaagaag gtacagggta
     6061 gccaagggct gaagggagcc cattggcatg gtggcgctag cagctggtac ccagcttcta
     6121 gagatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
     6181 ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt ttggaaagtc ccgttgattt
     6241 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     6301 tcaaaccgct atccacgccc attggtgtac tgccaaaacc gcatcaccat ggtaatagcg
     6361 atgactaata cgtagatgta ctgccaagta ggaaagtccc gtaaggtcat gtactgggca
     6421 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcggact tggcatatga
     6481 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     6541 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     6601 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     6661 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     6721 caatgtcaac atggcggtca tattggacat gagccaatat aaatgtacat attatgatat
     6781 agatacaacg tatgcaatgg ccaatagcca atattgattt atgctatata accaatgact
     6841 aatatggcta attgccaata ttgattcaat gtatagatct tccataccta ccagttctgc
     6901 gcctgcagca atggcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga
     6961 attggtcgac tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg
     7021 tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg
     7081 ccatccaaaa accatgggtc tgtctgctca gtccagtcgt ggacctgacc ccacgcaacg
     7141 cccaaaataa taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac
     7201 ccacggggcc agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     7261 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     7321 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     7381 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     7441 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccgatccgg
     7501 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     7561 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     7621 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     7681 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
     7741 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     7801 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     7861 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     7921 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     7981 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     8041 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     8101 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     8161 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     8221 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
     8281 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     8341 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     8401 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     8461 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
     8521 cccgggatct gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggtg
     8581 ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tcggaccgcg ccgccccgac
     8641 tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata
     8701 gaactaaaga catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg
     8761 gccacgggga tgaagcaggg cggcacctcg ctaacggatt caccactcca agaattggag
     8821 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
     8881 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc ggggccgacg cgctgggcta
     8941 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     9001 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     9061 ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9541 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     9601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10201 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10501 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10861 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10921 taggcgtatc acgaggccct ttcgtcttca agaattctca tgtttgacag cttatcatcg
    10981 ataagctgat cctcacaggc cgcacccagc ttttcttccg ttgccccagt agcatctctg
    11041 tctggtgacc ttgaagagga agaggagggg tcccgagaat ccccatccct accgtccagc
    11101 aaaaaggggg acgaggaatt tgaggcctgg cttgaggctc aggacgcaaa tcttgaggat
    11161 gttcagcggg agttttccgg gctgcgagta attggtgatg aggacgagga tggttcggag
    11221 gatggggaat tttcagacct ggatctgtct gacagcgacc atgaagggga tgagggtggg
    11281 ggggctgttg gagggggcag gagtctgcac tccctgtatt cactgagcgt cgtctaataa
    11341 agatgtctat tgatctcttt tagtgtgaat catgtctgac gaggggccag gtacaggacc
    11401 tggaaatggc ctaggagaga agggagacac atctggacca gaaggctccg gcggcagtgg
    11461 acctcaaaga agagggggtg ataaccatgg acgaggacgg ggaagaggac gaggacgagg
    11521 aggcggaaga ccaggagccc cgggcggctc aggatcaggg ccaagacata gagatggtgt
    11581 ccggagaccc caaaaacgtc caagttgcat tggctgcaaa gggacccacg gtggaacagg
    11641 agcaggagca gg
//
