LOCUS       sequence-23970-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     Drapkin Lab Plasmids
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-HE4
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(415..437)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(578..596)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aannncccnn nanntcggat ctgatcgaag gtcgtgggat ccggtcaggc acaggagcag
       61 agaagactgg cgtgtgcccc gagctccagg ctgaccagaa ctgcacgcaa gagtgcgtct
      121 cggacagcga atgcgccgac aacctcaagt gctgcagcgc gggctgtgcc accttctgct
      181 ctctgcccaa tgataaggag ggttcctgcc cccaggtgaa cattaacttt ccccagctcg
      241 gcctctgtcg ggaccagtgc caggtggaca gccagtgtcc tggccagatg aaatgctgcc
      301 gcaatggctg tgggaaggtg tcctgtgtca ctcccaattt ctgaccggat ccccgggaat
      361 tcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
      421 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      481 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
      541 acgtagcgat agcggagtaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      601 taggttaatg tcatgataat aatgtttncn ttaaacgntc agggnggcac ttttcgggga
      661 aatgtgcgcg ggaaccccct attttgttta nttttnnnnn tacnttcaaa tttgtatccc
      721 cnccatgaac aaanacnccc ctgataancn ncttcaataa tatngaaaaa ggaagagtat
      781 gagtattcaa catttccgtg tcgcccttat tc
//
