LOCUS       sequence-239700-       11694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate 
            (containing SOSIP mutations); C-terminal truncation; QES mutant for 
            enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*
FEATURES             Location/Qualifiers
     source          1..11694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        796..900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(901..969,970..1761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1119..1138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1932..2520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2421..2440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2951..3096
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3139..4176
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4174..4193)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4218..4266
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4920..5299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5300..5503
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5454..5474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5500..5524
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7824..7958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7848..7867)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7902..7921
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8101..8123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8683..10472
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
       61 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
      121 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
      181 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
      241 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
      301 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
      361 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
      421 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
      481 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
      541 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
      601 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      661 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1141 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1441 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2041 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2581 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2641 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     2701 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     2761 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     2821 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     2881 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     2941 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     3001 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     3061 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     3121 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     3181 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     3241 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     3301 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     3361 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     3421 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     3481 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     3541 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     3601 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     3661 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     3721 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     3781 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     3841 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     3901 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     3961 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     4021 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     4081 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     4141 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     4201 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     4261 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4321 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4381 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     4441 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     4501 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     4561 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     4621 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     4681 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     4741 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     4801 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4861 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4921 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5161 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5221 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     5281 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     5341 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5401 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     5461 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5521 gatctctaga agctgggtac cagctgctag cgccaccatg ccaatgggct cccttcagcc
     5581 cttggctacc ctgtaccttc ttgggatgtt ggtcgcatct gtgcttgccg cagaaaactt
     5641 gtgggtaact gtctactatg gagtacccgt ttggaaagac gcagaaacaa cgcttttttg
     5701 cgcatccgac gcgaaggcat acgaaacgga aaagcacaat gtttgggcaa cgcatgcctg
     5761 cgtcccgaca gacccaaatc ctcaggagat tcaccttgag aatgtaacag aggaattcaa
     5821 catgtggaag aataacatgg tagaacaaat gcatacggat attatcagtc tttgggacca
     5881 gagccttaaa ccttgcgtta aacttacgcc cctctgcgta actctgcaat gtactaacgt
     5941 aacgaataac attacggacg acatgcgcgg cgaactgaag aattgcagct ttaatatgac
     6001 aacggagttg cgagataaaa agcagaaggt gtacagcctc ttctacaggc ttgacctcgt
     6061 gcaaataaac gaaaaccagg ggaacaggag caataactcc aataaagaat acagattgat
     6121 caactgtaac actagcgaga taacccaggc ctgcccgaag gtgtcatttg agccgattcc
     6181 tatacactat tgcgccccgg cagggtatgc gatcttgaaa tgcaaggata agaagtttaa
     6241 tggaactggt ccctgtccca gtgtgagtac ggttcagtgt acccacggca taaagccagt
     6301 gatgtccact cagcttctcc tcaatggcag cctcgcagag gaagaggtca tgatacggag
     6361 tgagaatatt actaacaatg ccaagaatat cttggtccaa tttaataccc ccgtccagat
     6421 taattgtacc cgacctaata ataataccag aaaatccatt cgcatcggac caggtcaggc
     6481 gttctatgcc acgggagata taataggtga catccgacag gcgcattgca atgtaagcaa
     6541 ggccacatgg aatgagactc tgggtaaagt tgtcaagcag ctcaggaagc atttcgggaa
     6601 caataccatc atccgatttg cgaacagtag tgggggtgat cttgaagtaa ctacacattc
     6661 atttaactgc ggcggggagt ttttttattg taacacatcc gggttgttca actccacttg
     6721 gatatctaac actagtgttc agggatcaaa ctccacagga tcaaacgata gtataacact
     6781 tccttgtcgc atcaaacaga ttatcaacat gtggcagcga atcggccagg cgatgtacgc
     6841 cccgcctatc cagggagtaa tacggtgcgt aagcaacata acgggcctta tacttacgcg
     6901 cgatggaggc agcactaatt ctaccactga gacgttccgg cctggcggtg gggacatgcg
     6961 agacaactgg cggtctgagt tgtggaagta caaagtggtt aaaatcgaac ccctgggtgt
     7021 cgccccaacg agatgtaaac gacgggtagt gggaaggagg agaaggcgac gggcggtcgg
     7081 cattggggcc gttttcttgg gttttctggg ggcggctggg agcactatgg gagcagctag
     7141 tatgaccctg accgtacagg cgaggaacta cctgtccggt atagtgcaac aacagtcaaa
     7201 ccttcttagg gctcctgagg cgcagcaaca tctccttaag ttgactgtct ggggcataaa
     7261 gcaactccaa gcgcgggttc tcgcggtgga aagatacctt agggaccaac aacttttggg
     7321 gatgtggggt tgttcaggca aactcatctg ttgcactaac gttccttgga actcctcatg
     7381 gtctaaccga aatctctccg agatctggga taatatgaca tggttgcagt gggataagga
     7441 gattagcaat tacactcaaa taatctatgg gctcctggag gaaagccaga atcagcagga
     7501 gaagaatgaa caggatcttc tggcccttga taagtgggca agtctttgga attggttcga
     7561 catttcaaat tggctttggt atataaagat atttataatg attgtcgggg gtttgatagg
     7621 tttgaggatt gtgtttgctg tgctgtctgt tatccacagg gttaggcagg ggtatagtcc
     7681 gctctctttt caaacccata cccccaaccc caggggattg gaccgacccg agcggattga
     7741 ggaggaagac ggtgaacagg accggggcag aagtacccgc cttgttagcg ggtttctgtg
     7801 ataactcgag gccggcaagg ccggatccag acatgataag atacattgat gagtttggac
     7861 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     7921 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     7981 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     8041 aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     8101 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     8161 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag
     8221 gtcgactcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg
     8281 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     8341 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     8401 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     8461 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     8521 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     8581 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     8641 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     8701 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     8761 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     8821 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     8881 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     8941 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     9001 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     9061 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     9121 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     9181 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     9241 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     9301 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     9361 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     9421 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     9481 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     9541 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     9601 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
     9661 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
     9721 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
     9781 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     9841 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     9901 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     9961 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
    10021 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
    10081 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
    10141 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
    10201 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
    10261 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    10321 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat
    10381 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg
    10441 ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt
    10501 aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg
    10561 ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc
    10621 caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca
    10681 acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt
    10741 aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc
    10801 tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac
    10861 cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct
    10921 gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac
    10981 ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag
    11041 cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt
    11101 aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt
    11161 actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac
    11221 gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga
    11281 acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc
    11341 ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat
    11401 cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata
    11461 atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg
    11521 agacccggat gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg
    11581 acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg
    11641 gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc ggcc
//
