LOCUS       sequence-239701-       11832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; 
            DS-SOSIP.4mut variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.DS-SOSIP.4mut-gp160
FEATURES             Location/Qualifiers
     source          1..11832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(488..506)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        574..678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(679..747,748..1539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(897..916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1710..2298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2199..2218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2729..2874
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2917..3954
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3952..3971)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3996..4044
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4698..5077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5078..5281
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5232..5252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5278..5302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7932..8066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7956..7975)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8010..8029
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8209..8231)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8791..10580
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac
       61 actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag
      121 actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag
      181 atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg
      241 cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc
      301 ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc
      361 ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg
      421 tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
      481 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      541 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      601 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      661 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      721 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      781 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      841 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      901 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      961 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1021 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1081 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1141 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1201 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     1261 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1321 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1381 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1441 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1501 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1561 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1621 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1681 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1741 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1801 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1861 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1921 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1981 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2041 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2101 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2161 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2221 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2281 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2341 tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     2401 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     2461 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc
     2521 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
     2581 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
     2641 gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
     2701 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
     2761 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     2821 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     2881 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
     2941 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     3001 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     3061 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     3121 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     3181 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     3241 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     3301 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     3361 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     3421 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     3481 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     3541 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     3601 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     3661 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     3721 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     3781 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     3841 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     3901 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
     3961 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     4021 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     4081 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     4141 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     4201 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
     4261 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
     4321 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
     4381 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
     4441 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     4501 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
     4561 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     4621 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     4681 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     4741 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4801 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4861 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4921 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     4981 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     5041 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     5101 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     5161 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     5221 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5281 tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagcg ccaccatgcc
     5341 aatgggctcc cttcagccct tggctaccct gtaccttctt gggatgttgg tcgcatctgt
     5401 gcttgccgca gaaaacttgt gggtaactgt ctactatgga gtacccgttt ggaaagacgc
     5461 agaaacaacg cttttttgcg catccgacgc gaaggcatac gaaacggaaa agcacaatgt
     5521 ttgggcaacg catgcctgcg tcccgacaga cccaaatcct caggagattc accttgagaa
     5581 tgtaacagag gaattcaaca tgtggaagaa taacatggta gaacaaatgc atacggatat
     5641 tatcagtctt tgggaccaga gccttaaacc ttgcgttaaa cttacgcccc tctgcgtaac
     5701 tctgcaatgt actaacgtaa cgaataacat tacggacgac atgcgcggcg aaatgaagaa
     5761 ttgcagcttt aatatgacaa cggagttgcg agataaaaag cagaaggtgt acagcctctt
     5821 ctacaggctt gacgttgtgc aaataaacga aaaccagggg aacaggagca ataactccaa
     5881 taaagaatac agattgatca actgtaacac tagcgcatgt acccaggcct gcccgaaggt
     5941 gtcatttgag ccgattccta tacactattg cgccccggca gggtttgcga tcttgaaatg
     6001 caaggataag aagtttaatg gaactggtcc ctgtcccagt gtgagtacgg ttcagtgtac
     6061 ccacggcata aagccagtgg tatccactca gcttctcctc aatggcagcc tcgcagagga
     6121 agaggtcatg atacggagtg agaatattac taacaatgcc aagaatatct tggtccaatt
     6181 taataccccc gtccagatta attgtacccg acctatgaat atgaccagaa aatccattcg
     6241 catcggacca ggtcaggcgt tctatgccct gggagatata ataggtgaca tccgacaggc
     6301 gcattgcaat gtaagcaagg ccacatggaa tgagactctg ggtaaagttg tcaagcagct
     6361 caggaagcat ttcgggaaca ataccatcat ccgatttgcg aacagtagtg ggggtgatct
     6421 tgaagtaact acacattcat ttaactgcgg cggggagttt ttttattgta acacatccgg
     6481 gttgttcaac tccacttgga tatctaacac tagtgttcag ggatcaaact ccacaggatc
     6541 aaacgatagt ataacacttc cttgtcgcat caaacagatt atcaacatgt ggcagcgaat
     6601 cggccagtgc atgtacgccc cgcctatcca gggagtaata cggtgcgtaa gcaacataac
     6661 gggccttata cttacgcgcg atggaggcag cactaattct accactgaga cgttccggcc
     6721 tggcggtggg gacatgcgag acaactggcg gtctgagttg tacaagtaca aagtggttaa
     6781 aatcgaaccc ctgggtgtcg ccccaacgag atgtaaacga cgggtagtgg gaaggaggag
     6841 aaggcgacgg gcggtcggca ttggggccgt tttcttgggt tttctggggg cggctgggag
     6901 cactatggga gcagctagta tgaccctgac cgtacaggcg aggaacctcc tgtccggtat
     6961 agtgcaacaa cagtcaaacc ttcttagggc tcctgaggcg cagcaacatc tccttaagtt
     7021 gactgtctgg ggcataaagc aactccaagc gcgggttctc gcggtggaaa gataccttag
     7081 ggaccaacaa cttttgggga tttggggttg ttcaggcaaa ctcatctgtt gcactaacgt
     7141 tccttggaac tcctcatggt ctaaccgaaa tctctccgag atctgggata atatgacatg
     7201 gttgcagtgg gataaggaga ttagcaatta cactcaaata atctatgggc tcctggagga
     7261 aagccagaat cagcaggaga agaatgaaca ggatcttctg gcccttgata agtgggcaag
     7321 tctttggaat tggttcgaca tttcaaattg gctttggtat ataaagatat ttataatgat
     7381 tgtcgggggt ttgataggtt tgaggattgt gtttgctgtg ctgtctgtta tccacagggt
     7441 taggcagggg tatagtccgc tctcttttca aacccatacc cccaacccca ggggattgga
     7501 ccgacccgag cggattgagg aggaagacgg tgaacaggac cggggcagaa gtacccgcct
     7561 tgttagcggg tttctggctc tggcgtggga cgacttgcgg agcctttgcc tcttctgcta
     7621 ccatcgcctc agagatttta tcttgatcgc tgccagaata gtagaattgt tgggccacag
     7681 ctccctcaaa ggtttgagac ttggatggga aggtcttaaa tacctgtgga atttgctggc
     7741 ttactggggt agggagctga agatttctgc cattaacttg ttcgacacaa tagctattgc
     7801 agttgcggag tggactgaca gagtcataga aataggccaa agattgtgtc gggcgtttct
     7861 ccacattcca cggcggatta ggcaaggact tgaacgggcc ctcctttagt aactcgaggc
     7921 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     7981 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     8041 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     8101 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     8161 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     8221 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     8281 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga
     8341 ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc
     8401 ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc
     8461 acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt
     8521 ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca
     8581 gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac
     8641 caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac
     8701 gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat
     8761 agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag
     8821 tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg
     8881 ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag
     8941 ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc
     9001 acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc
     9061 ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt
     9121 atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg
     9181 ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat
     9241 aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg
     9301 gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca
     9361 taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac
     9421 aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag
     9481 cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat
     9541 ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac
     9601 gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt
     9661 ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg
     9721 gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg
     9781 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc
     9841 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac
     9901 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc
     9961 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
    10021 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
    10081 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
    10141 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc
    10201 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa
    10261 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
    10321 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
    10381 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
    10441 tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt
    10501 agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt
    10561 cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg
    10621 ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg
    10681 agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat
    10741 gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg
    10801 atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag
    10861 ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc
    10921 ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa
    10981 ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt
    11041 aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat
    11101 atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg
    11161 tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg
    11221 tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata
    11281 tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc
    11341 caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc
    11401 acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg
    11461 tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg
    11521 cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc
    11581 tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga
    11641 tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct
    11701 ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact
    11761 acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc
    11821 ccggcctcca cc
//
