LOCUS       sequence-239704-       12047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate 
            (containing SOSIP mutations); QES mutant for enhanced presentation 
            of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-gp160-QES.i05.c06
FEATURES             Location/Qualifiers
     source          1..12047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(896..964,965..1756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1114..1133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1927..2515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2946..3091
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3134..4171
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4169..4188)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4213..4261
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4915..5294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5295..5498
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5449..5469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5495..5519
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8149..8283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8173..8192)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8227..8246
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8426..8448)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9008..10797
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg
       61 gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc
      121 tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg
      181 tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc
      241 aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc
      301 tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc
      361 ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat
      421 cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa
      481 catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt
      541 tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag
      601 acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta
      661 tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg
     2581 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     2641 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     2701 gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     2761 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     2821 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg
     2881 cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt
     2941 ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc
     3001 agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     3061 acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg
     3121 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     3181 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     3241 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     3301 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     3361 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     3421 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     3481 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     3541 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     3601 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     3661 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     3721 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     3781 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     3841 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     3901 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     3961 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     4021 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     4081 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     4141 tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4201 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     4261 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4321 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4381 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg
     4441 ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg
     4501 gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg
     4561 gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa
     4621 acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa
     4681 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     4741 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     4801 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     4861 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt
     4921 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5161 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5221 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
     5281 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
     5341 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5401 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
     5461 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct
     5521 ctagaagctg ggtaccagct gctagcgcca ccatgccaat gggctccctt cagcccttgg
     5581 ctaccctgta ccttcttggg atgttggtcg catctgtgct tgccgcagaa aacttgtggg
     5641 taactgtcta ctatggagta cccgtttgga aagacgcaga aacaacgctt ttttgcgcat
     5701 ccgacgcgaa ggcatacgaa acggaaaagc acaatgtttg ggcaacgcat gcctgcgtcc
     5761 cgacagaccc aaatcctcag gagattcacc ttgagaatgt aacagaggaa ttcaacatgt
     5821 ggaagaataa catggtagaa caaatgcata cggatattat cagtctttgg gaccagagcc
     5881 ttaaaccttg cgttaaactt acgcccctct gcgtaactct gcaatgtact aacgtaacga
     5941 ataacattac ggacgacatg cgcggcgaac tgaagaattg cagctttaat atgacaacgg
     6001 agttgcgaga taaaaagcag aaggtgtaca gcctcttcta caggcttgac ctcgtgcaaa
     6061 taaacgaaaa ccaggggaac aggagcaata actccaataa agaatacaga ttgatcaact
     6121 gtaacactag cgagataacc caggcctgcc cgaaggtgtc atttgagccg attcctatac
     6181 actattgcgc cccggcaggg tatgcgatct tgaaatgcaa ggataagaag tttaatggaa
     6241 ctggtccctg tcccagtgtg agtacggttc agtgtaccca cggcataaag ccagtgatgt
     6301 ccactcagct tctcctcaat ggcagcctcg cagaggaaga ggtcatgata cggagtgaga
     6361 atattactaa caatgccaag aatatcttgg tccaatttaa tacccccgtc cagattaatt
     6421 gtacccgacc taataataat accagaaaat ccattcgcat cggaccaggt caggcgttct
     6481 atgccacggg agatataata ggtgacatcc gacaggcgca ttgcaatgta agcaaggcca
     6541 catggaatga gactctgggt aaagttgtca agcagctcag gaagcatttc gggaacaata
     6601 ccatcatccg atttgcgaac agtagtgggg gtgatcttga agtaactaca cattcattta
     6661 actgcggcgg ggagtttttt tattgtaaca catccgggtt gttcaactcc acttggatat
     6721 ctaacactag tgttcaggga tcaaactcca caggatcaaa cgatagtata acacttcctt
     6781 gtcgcatcaa acagattatc aacatgtggc agcgaatcgg ccaggcgatg tacgccccgc
     6841 ctatccaggg agtaatacgg tgcgtaagca acataacggg ccttatactt acgcgcgatg
     6901 gaggcagcac taattctacc actgagacgt tccggcctgg cggtggggac atgcgagaca
     6961 actggcggtc tgagttgtgg aagtacaaag tggttaaaat cgaacccctg ggtgtcgccc
     7021 caacgagatg taaacgacgg gtagtgggaa ggaggagaag gcgacgggcg gtcggcattg
     7081 gggccgtttt cttgggtttt ctgggggcgg ctgggagcac tatgggagca gctagtatga
     7141 ccctgaccgt acaggcgagg aactacctgt ccggtatagt gcaacaacag tcaaaccttc
     7201 ttagggctcc tgaggcgcag caacatctcc ttaagttgac tgtctggggc ataaagcaac
     7261 tccaagcgcg ggttctcgcg gtggaaagat accttaggga ccaacaactt ttggggatgt
     7321 ggggttgttc aggcaaactc atctgttgca ctaacgttcc ttggaactcc tcatggtcta
     7381 accgaaatct ctccgagatc tgggataata tgacatggtt gcagtgggat aaggagatta
     7441 gcaattacac tcaaataatc tatgggctcc tggaggaaag ccagaatcag caggagaaga
     7501 atgaacagga tcttctggcc cttgataagt gggcaagtct ttggaattgg ttcgacattt
     7561 caaattggct ttggtatata aagatattta taatgattgt cgggggtttg ataggtttga
     7621 ggattgtgtt tgctgtgctg tctgttatcc acagggttag gcaggggtat agtccgctct
     7681 cttttcaaac ccataccccc aaccccaggg gattggaccg acccgagcgg attgaggagg
     7741 aagacggtga acaggaccgg ggcagaagta cccgccttgt tagcgggttt ctggctctgg
     7801 cgtgggacga cttgcggagc ctttgcctct tctgctacca tcgcctcaga gattttatct
     7861 tgatcgctgc cagaatagta gaattgttgg gccacagctc cctcaaaggt ttgagacttg
     7921 gatgggaagg tcttaaatac ctgtggaatt tgctggctta ctggggtagg gagctgaaga
     7981 tttctgccat taacttgttc gacacaatag ctattgcagt tgcggagtgg actgacagag
     8041 tcatagaaat aggccaaaga ttgtgtcggg cgtttctcca cattccacgg cggattaggc
     8101 aaggacttga acgggccctc ctttagtaac tcgaggccgg caaggccgga tccagacatg
     8161 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     8221 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     8281 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     8341 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg
     8401 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8461 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8521 gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga
     8581 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     8641 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     8701 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     8761 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     8821 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     8881 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     8941 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     9001 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     9061 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     9121 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     9181 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     9241 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     9301 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     9361 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     9421 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     9481 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     9541 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     9601 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     9661 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     9721 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     9781 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     9841 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     9901 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     9961 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
    10021 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
    10081 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
    10141 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
    10201 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
    10261 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
    10321 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
    10381 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
    10441 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
    10501 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
    10561 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
    10621 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
    10681 atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt
    10741 tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgctg
    10801 gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt
    10861 gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg
    10921 agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac
    10981 aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt
    11041 tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc
    11101 actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat
    11161 caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat
    11221 cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa
    11281 ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga
    11341 aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc
    11401 caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc
    11461 gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag
    11521 ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg
    11581 caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc
    11641 cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg
    11701 ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc
    11761 caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc
    11821 tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc
    11881 tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc
    11941 tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac
    12001 tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctcca
//
