LOCUS       sequence-239706-       11610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp160-754*
FEATURES             Location/Qualifiers
     source          1..11610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(605..623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        691..795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(796..864,865..1656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1014..1033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1827..2415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2316..2335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2846..2991
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3034..4071
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4069..4088)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4113..4161
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4815..5194
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5195..5398
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5349..5369
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5395..5419
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7701..7835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7725..7744)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7779..7798
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7978..8000)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8560..10349
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
       61 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
      121 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
      181 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
      241 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
      301 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
      361 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
      421 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
      481 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
      541 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
      601 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      661 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      781 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      841 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      901 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      961 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1021 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     1081 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1141 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1201 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1261 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1321 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1381 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1441 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1501 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1561 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1621 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1681 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1741 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1801 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1861 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1921 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1981 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2041 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2101 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2161 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2221 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2281 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2341 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2401 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2461 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     2521 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     2581 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc
     2641 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     2701 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     2761 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
     2821 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
     2881 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
     2941 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
     3001 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
     3061 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     3121 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     3181 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     3241 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     3301 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     3361 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     3421 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     3481 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     3541 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     3601 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     3661 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     3721 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     3781 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     3841 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     3901 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     3961 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     4021 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     4081 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     4141 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     4201 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     4261 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     4321 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     4381 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     4441 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     4501 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     4561 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     4621 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     4681 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     4741 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     4801 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     4861 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     4921 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4981 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5041 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     5101 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     5161 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     5221 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5281 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     5341 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     5401 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcccat
     5461 gggttccctc caaccacttg caacgcttta tctgctcggc atgcttgtag cgtcagtgct
     5521 tgcaaatctc gacctttggg tcaccgtata ctacggagtc cccgtgtgga aagaggctaa
     5581 gacgaccctg ttttgtgcgt cagacgcgaa agcgtatgac aaggaagtgc ataacgtgtg
     5641 ggcaacacac gcatgtgttc cgacggaccc taacccgcag gaaatcgtcc ttgaaaacgt
     5701 gaccgaaaac ttcaacatgt ggaagaacga catggttgac caaatgcacg aggacataat
     5761 cagtctttgg gatcaatcct tgaagccctg tgtgaagctg acgcctttgt gcgtaacttt
     5821 gaactgtaaa aacgtaaata tttcagcgaa tgctaacgcg accgctacac ttaattcaag
     5881 tatgaacggc gaaataaaga attgctcctt caacaccacg acagaactta gagataaaaa
     5941 gcaaaaagtc tacgcccttt tctataaacc ggatgtcgtg ccacttaacg gtggtgagca
     6001 caatgagaca ggtgagtaca ttctcatcaa ctgcaattca tctacaataa cgcaagcatg
     6061 tccgaaagtt tccttcgatc cgatccctat tcactactgc gctcctgcag ggtacgccat
     6121 actgaagtgt aacaataaaa cgtttaacgg aacaggccct tgcaataatg taagtactgt
     6181 ccagtgcacg catggcataa aacccgtcgt gagcacacaa cttcttctta acggatcact
     6241 cgccgaggaa gagatcatcg tgcgaagtga gaacttgacg aataatataa agacgatcat
     6301 agtccatctc aacaagtccg tcgagattaa atgcactagg cccaacaaca atacgcggaa
     6361 atccgtacgg ataggtcctg gacaaacatt ctatgctacc ggtgagatta taggtgatat
     6421 acgcgaagct cattgcaata tcagtaggga gacctggaat tccactttga tccaagtgaa
     6481 agaaaaactg cgggagcatt acaacaaaac gatcaaattt gaaccttctt ctggaggtga
     6541 cctggaggtt acgacgcaca gttttaactg taggggagaa ttcttctact gcgatacgac
     6601 caagctgttc aacgagacta agctgtttaa cgagtcagaa tacgttgaca ataagactat
     6661 tatcttgccc tgccggataa agcaaattat caatatgtgg caagaagtgg gaagggcaat
     6721 gtacgcgccg ccgattgaag ggaatattac ttgcaagagc aacataactg gtcttcttct
     6781 cacatgggat ggtggagaga acagtaccga aggcgttttt aggcctggcg gtggcaatat
     6841 gaaggataac tggcgctcag aactctataa gtacaaagtc gtcgaaataa agcccttggg
     6901 cgttgcgcct acgaaaagta aacgaaaagt agtagggagg gaaaagcgag cggttggctt
     6961 gggtgcagtg cttctcgggt tcctgggagc tgcggggtca acaatgggag ctgcttccat
     7021 aaccttgact gtgcaggcaa ggcagctgct gagtgggata gtgcagcaac aatccaatct
     7081 gcttagggca attgaagctc agcaacatct tttgcagctc acagtctggg gcataaagca
     7141 actgcaaacc agagtcctcg cgattgagcg gtatcttaag gaccagcaac tgctgggact
     7201 ctgggggtgt tccgggaagc ttatttgtgc cactgcggta ccatggaact cctcttggtc
     7261 caacaagtct ctgggcgata tttgggacaa tatgacgtgg atgcagtggg atcgcgagat
     7321 atccaactac accaacacga tcttccggct tctcgaagac agtcagaatc aacaagagaa
     7381 gaatgagaag gatctgttgg cccttgacag ctggaaaaat ctttggaatt ggtttgacat
     7441 tactaactgg ctgtggtaca taaaaatctt cataatgatt gtcggtggcc ttatcggtct
     7501 ccgcatcata ttcggggtat tggctatagt caaaagggta cgccaggggt atagcccgtt
     7561 gagtttccaa acgctcattc cgaatccgcg aggtcctgat cgcttgggac ggatcgaaga
     7621 ggaaggaggt gaacaagaca aggatcgatc catacgactg gtctctgggt ttctttagta
     7681 actcgaggcc ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa
     7741 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7801 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     7861 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     7921 gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7981 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8041 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc
     8101 gactctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc
     8161 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg
     8221 ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt
     8281 gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca
     8341 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag
     8401 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc
     8461 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc
     8521 attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc
     8581 gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg
     8641 aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca
     8701 ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc
     8761 attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc
     8821 ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca
     8881 gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt
     8941 tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc
     9001 ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc
     9061 catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg
     9121 ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg
     9181 gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga
     9241 cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc
     9301 cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag
     9361 tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg
     9421 taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa
     9481 ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg
     9541 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg
     9601 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt
     9661 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     9721 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     9781 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9841 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     9901 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca
     9961 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg
    10021 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
    10081 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
    10141 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
    10201 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg
    10261 atacccagta gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc
    10321 gtgaattttc gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag
    10381 gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt
    10441 tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa
    10501 ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca
    10561 gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat
    10621 acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc
    10681 atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat
    10741 aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat
    10801 attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc
    10861 agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg
    10921 gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag
    10981 acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact
    11041 acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg
    11101 actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc
    11161 tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg
    11221 accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc
    11281 tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc
    11341 ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga
    11401 cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc
    11461 tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg
    11521 gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc
    11581 tcctcgaccc cggcctccac ctcctgctcc
//
