LOCUS       sequence-239707-       11596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q769.d22 HIV-1 
            isolate; C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q769gp160-754*
FEATURES             Location/Qualifiers
     source          1..11596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(683..701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        769..873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(874..942,943..1734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1092..1111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1905..2493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2394..2413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2924..3069
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3112..4149
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4147..4166)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4191..4239
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4893..5272
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5273..5476
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5427..5447
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5473..5497
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             7161..7169
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7785..7919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7809..7828)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7863..7882
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8062..8084)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8644..10433
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc
       61 cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc
      121 cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct
      181 ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg
      241 tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga
      301 atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg
      361 gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc
      421 atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg
      481 agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga
      541 ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac
      601 ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat
      661 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      721 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      961 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1021 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1081 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     1141 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1201 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1261 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1321 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1381 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     1441 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1501 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1561 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1621 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1681 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1741 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2521 ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     2581 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     2641 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg
     2701 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     2761 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     2821 atccgttagc gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc
     2881 ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc
     2941 cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag
     3001 gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga
     3061 cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag
     3121 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     3181 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     3241 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     3301 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     3361 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     3421 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     3481 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     3541 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     3601 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     3661 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     3721 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     3781 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     3841 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     3901 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     3961 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     4021 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     4081 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag
     4141 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     4201 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4261 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4321 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4381 tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat
     4441 ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca
     4501 cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta
     4561 ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc
     4621 accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc
     4681 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa
     4741 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     4801 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
     4861 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
     4921 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4981 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     5041 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     5101 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     5161 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     5221 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     5281 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     5341 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5401 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     5461 ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc
     5521 tagcgccacc atgcccatgg ggtctttgca acccctcgct actctttatt tgctgggtat
     5581 gttggtagcg agcgttctgg cggcgggcaa tctctgggta accgtgtact acggcgtgcc
     5641 agtgtggcgg gatgctgaaa caaccctctt ttgtgcaagt gatgcaaaag cgtatgaccg
     5701 agaagcacac aatgtttggg ctacacatgc gtgcgttccg acagatccat ccccccaaga
     5761 agtaccattg gggaatgtaa ccgaggaatt caacatgtgg aagaataaca tggttgaaca
     5821 gatgcataca gacatcatct ctttgtggga ccagtcactg cagccgtgtg tgaaacttac
     5881 gcctctttgt gtcacgctga attgttccaa tagtaataac attccaagtg tcagcaacat
     5941 taccgatgat atgaaagaag agattaagaa ctgcagcttt aacatgacaa ccgaattgaa
     6001 ggataaaaag cagaacgttt actctttgtt ctatcgactg gacgttgtac ccctcgaaac
     6061 aaaaaccaac cagaacagta gtcactcaag gtatcggctc attaactgca acacgtctgc
     6121 catcacgcag gcttgcccaa aagtttcttt cgagccgatc cctattcact attgtgcgcc
     6181 tgcaggcttt gctatcctga aatgtaacga caagggcttt aacggcactg gactttgtaa
     6241 aaacgtctca acagtacaat gcacgcatgg gattaaaccg gtcgtcagta cccagctgct
     6301 ccttaatgga agccttgccg agggtaaggt aatggtacga tccgaaaata ttacaaataa
     6361 tgcgaaaaac attataatac agtttaacaa ctctgtgcaa attaattgca cgcgcccagg
     6421 taataacacg cgaaaaagta tacatctcgg gcctgggaaa gttttttacg cgacggacat
     6481 aataggggat ataaggaaag cgcactgtaa tgttaatcgc caacaatgga ataagaccct
     6541 tcaggatgtg gcaacgcaac tcaggaccca cttcagaaac cgtacgatca tatttaataa
     6601 tagtctcggt ggggatttgg aaataactac gcatagtttt aattgtaggg gagagttttt
     6661 ctattgtaac acttccgggc tctttaatgg gatatggaac ggaacgcaag aacctaatcg
     6721 gaccgagtct aacgatacaa ttacgcttca atgtcggata aagcagatca tcaacatgtg
     6781 gcagagagta ggccaggcaa tttacgcacc ccctatccag ggggagatca gatgcgaaag
     6841 taacataacc ggacttatcc tgacgaggga tggggggatc atcaacagta cagaggaaac
     6901 gtttagacct gggggcgggg acatgagaga taattggagg tccgagctct ataaatacaa
     6961 agttgtaaag atagaaccgc ttggagtggc tccaactaag gccaagagac gggtcgtgga
     7021 gagagaaaaa cgggcggtag gatttggcgc gttctttctc ggtttccttg gcgcagccgg
     7081 atcaacgatg ggggccgcct ccatcaccct gacggttcag gcgcgccagc ttttgagtgg
     7141 gattgtgcag cagcagaata acctccttag ggccatagag gctcagcagc acctgctgaa
     7201 gcttaccgtt tggggcatta agcaactgca ggcgcgggta ctcgcggttg aaagatacct
     7261 taaggatcag caactgctgg gaatttgggg atgtagtgga aagttcatct gcacaaccac
     7321 ggtcccatgg aattcctcat ggtcaaataa atcacagtca gaaatatggg ataatatgac
     7381 gtggatgcag tgggacaagg agattaacaa ttacacccag attatctacg atcttattga
     7441 ggagtcacaa aggcaacaag agaaaaacga acaggacttg ctggcgctcg acaaatgggc
     7501 taatttgtgg aactggttcg acatcagtaa ctggctgtgg tacataaaga ttttcataat
     7561 gatagttggc gggctcatag ggcttcggat tgcgttcgca gtgctttctg tcatcaatag
     7621 ggtcaggcaa ggttattccc cgctctcctt tcaaacccat accccaaacc ctcgggattt
     7681 ggataggcca ggccggatag aggaggaagg aggtgagcag gatcgggacc gaagcataag
     7741 actggtctct gggtttcttt gataactcga ggccggcaag gccggatcca gacatgataa
     7801 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     7861 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     7921 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     7981 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg
     8041 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8101 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8161 gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta
     8221 aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt
     8281 ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca
     8341 caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca
     8401 ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc
     8461 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag
     8521 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac
     8581 cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc
     8641 ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt
     8701 caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt
     8761 cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc
     8821 aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga
     8881 gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat
     8941 agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc
     9001 aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac
     9061 accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc
     9121 tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc
     9181 atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg
     9241 ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt
     9301 tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac
     9361 acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc
     9421 actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg
     9481 gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg
     9541 cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta
     9601 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact
     9661 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca
     9721 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct
     9781 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9841 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     9901 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9961 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct
    10021 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc
    10081 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc
    10141 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
    10201 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
    10261 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
    10321 tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca
    10381 tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg
    10441 ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc
    10501 accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg
    10561 acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga
    10621 cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt
    10681 gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc
    10741 ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc
    10801 ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc
    10861 aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc
    10921 cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata
    10981 acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa
    11041 ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct
    11101 taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc
    11161 ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat
    11221 gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt
    11281 tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc
    11341 atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc
    11401 ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc
    11461 tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg
    11521 cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc
    11581 tctttcacgt cctcta
//
