LOCUS       sequence-23971-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     Drapkin Lab Plasmids
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HE4
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(522..540)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(523..540)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(560..577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    566..677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggagnnnta gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc catagcacca
      121 tgcctgcttg tcgcctaggc ccgctagccg ccgccctcct cctcagcctg ctgctgttcg
      181 gcttcaccct agtctcaggc acaggagcag agaagactgg cgtgtgcccc gagctccagg
      241 ctgaccagaa ctgcacgcaa gagtgcgtct cggacagcga atgcgccgac aacctcaagt
      301 gctgcagcgc gggctgtgcc accttctgct ctctgcccaa tgataaggag ggttcctgcc
      361 cccaggtgaa cattaacttt ccccagctcg gcctctgtcg ggaccagtgc caggtggaca
      421 gccagtgtcc tggccagatg aaatgctgcc gcaatggctg tgggaaggtg tcctgtgtca
      481 ctcccaattt ctgactcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
      541 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggggggtgg
      721 gcnagacaca ggggaagatg gnaaaaaata cagnatgctg ggangcgtgg ctctatgntt
      781 ctnagcgaaa aacagtgggn ttagggtncc ncccctgnan gccatancng ngggg
//
