LOCUS       sequence-23977-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Swarbrick A, Roy E, Allen T, Bishop JM
  TITLE     Id1 cooperates with oncogenic Ras to induce metastatic mammary 
            carcinoma by subversion of the cellular senescence response.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7.
  PUBMED    18378907
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Apr 8. 105(14):5402-7."
COMMENT     Sequence Label: pRQ-hRasV12
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..59
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(98..631,632..634,635..667))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 gaaacncctc cctacccggg tagaattcca catcaccact ttgtacaaga aagctgggtc
       61 tagatatctc gagtgcggcc gcgaattcgt tggccgctca ggagagcaca cacttgcagc
      121 tcatgcagcc ggggccactc tcatcaggag ggttcagctt ccgcagcttg tgctgccgga
      181 tctcacgcac caacgtgtag aaggcatcct ccactccctg ccgggtcttg gccgaggtct
      241 cgatgtaggg gatgccgtag cttcgggcga ggtcctgagc ctgccgagat tccacagtgc
      301 gtgcagccag gtcacacttg ttccccacca gcaccatggg cacgtcatcc gagtccttca
      361 cccgtttgat ctgctccctg tactggtgga tgtcctcaaa agacttggtg ttgttgatgg
      421 caaacacaca caggaagccc tccccggtgc gcatgtactg gtcccgcatg gcgctgtact
      481 cctcctggcc ggcggtatcc aggatgtcca acaggcacgt ctccccatca atgaccacct
      541 gcttccggta ggaatcctct atagtggggt cgtattcgtc cacaaaatgg ttctggatca
      601 gctggatggt cagcgcactc ttgcccacac cgacggcgcc caccaccacc agcttatatt
      661 ccgtcatgaa ttcggtaccc ggatccagtc gactgaattg gttcctttta aagagcctgc
      721 tttttttgta caaacttgtg aatgtgtcca ggcgatntga cggttcacta aaa
//
