LOCUS       sequence-239980-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs182222907 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            C2CD4AB-...
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        87..162
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             582..595
                     /estimated_length=14
     gap             764..773
                     /estimated_length=10
     gap             775..784
                     /estimated_length=10
ORIGIN
        1 ttnnnnnnan tnnaannnat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
       61 acaccgtagg tactatactg ttgatggttt tagagctaga aatagcaagt taaaataagg
      121 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttga attcgctagc
      181 taggtcttga aaggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
      241 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
      301 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
      361 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
      421 gacgtgacaa acggaanccg cacgnctcac tagtacnctc ncagacggac agcgccnnna
      481 ncnnngnnnn cgcgncnann gngatgnnnt gnggncnana nnnnntgctc nnnangnnnn
      541 nncnagagnn ncnnnngnga anggnngnng ngagnangnn gnnnnnnnnn nnnnnannnn
      601 nnnancnnan agncnnnnnn gnnanngtnt nnnnnnnnnt ctnnannann nnnnntgnnn
      661 nannctnann nnnnngtgan nnnntnnntg tcaagancgn ngnnnggcgn ncanntnnnn
      721 nannannnan nnnangnnnn cnnnannnnn nnnctnnnnn nngnnnnnnn nnngnnnnnn
      781 nnnnanna
//
