LOCUS       sequence-239998-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs17205526 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            C2CD4AB-...
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        114..189
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tnnngnnttn nnngnactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
       61 ctttatatat cttgtggaaa ggacgaaaca ccgtgttagc atcaaggcct caagttttag
      121 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      181 agtcggtgct tttttgaatt cgctagctag gtcttgaaag gggttggggt tgcgcctttt
      241 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
      301 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
      361 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
      421 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
      481 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
      541 ggccaatagc ggctgctcag cagggcgcgc cnagannnnn ggccggnaan gnnnnnngcn
      601 nnnggc
//
