LOCUS       sequence-240000-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs17205526 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            C2CD4AB-...
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        108..183
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             631..643
                     /estimated_length=13
     gap             670..680
                     /estimated_length=11
     gap             709..723
                     /estimated_length=15
     gap             832..846
                     /estimated_length=15
ORIGIN
        1 tttnaaangn actatnntat ncttaccgta acttgaaagt atttcgattt cttggcttta
       61 tatatcttgt ggaaaggacg aaacaccgaa gttaagaaca tcctttggtt ttagagctag
      121 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      181 tgcttttttg aattcgctag ctaggtcttg aaaggggttg gggttgcgcc ttttccaagg
      241 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      301 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      361 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      421 cttgcggttc gcggcgtgcc ggacgtgacn aacggaagcc gcacgtctca ctattaccct
      481 cgcanacgga cagcgccagg gagcaatggc ngcgcgccga ccgcgatgnn ctgngnncna
      541 tancggntgn nnnncannnn nnnccnanan nanngnncgg nanngnnnnn ngcgnnannn
      601 nnnnntgtgt nncnnnannn tnnttnannt nnnnnnnnnn nnngnannnt cnngatatnn
      661 nnnnnctntn nnnnnnnnnn gnnctnnnnn annnnnnnnt cntaaacann nnnnnnnnnn
      721 nnncnnnnnn ntgngngtnn nannnnntat ntnnnnnttt nnccnnnncn nnnnaatatn
      781 nnncncnnnn nnngnnnann nnnnnctnna nanatnttaa nanagttnca annnnnnnnn
      841 nnnnnnattt nnnntccntn tcnnngannn cnnntnttnn natnttttnn nnc
//
