LOCUS       sequence-240006-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            VPS13C-T...
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..22
                     /estimated_length=21
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             619..628
                     /estimated_length=10
ORIGIN
        1 annnnnnnnn nnnnnnnnnn nntntnntgn gnaaaggacg aaacaccgat ggtggcgctg
       61 aggcacagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggggttg
      181 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
      241 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
      301 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
      361 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
      421 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
      481 ccgcgatggg ctgtggccaa tancggctgc tcagcanggc gcgccgagag cagcggccgg
      541 naagggncgg tgcgngaggc ggnnngngnn ncgntnnnnt ccncnngnnt nnnnnnnnan
      601 nnnntnnnnn nntnnntann nnnnnnnntg angnnnngtc nnnnntgtnn ntnagatgan
      661 tnnntatnnn tnnnnntgnn nnnntnancn nnnnannnan nnaanncnnn nnnnnncnnn
      721 ntgtnnnnnn gg
//
