LOCUS       sequence-240030-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs1401419 (CRY2 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs140141...
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..30
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        110..185
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             622..637
                     /estimated_length=16
     gap             674..686
                     /estimated_length=13
     gap             717..729
                     /estimated_length=13
     gap             744..756
                     /estimated_length=13
     gap             763..780
                     /estimated_length=18
     gap             796..809
                     /estimated_length=14
ORIGIN
        1 tttnnaatng gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
       61 atatatcttg tggaaaggac gaaacaccgc tctgtttgct attggcctcg ttttagagct
      121 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      181 ggtgcttttt tgaattcgct agctaggtct tgaaaggggt tggggttgcg ccttttccaa
      241 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
      301 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
      361 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
      421 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
      481 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
      541 aatancggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcggnag
      601 gcggtgtgtg nnncgnnnnn gnnnnnnnnn nnnnnnnann nnccnngcnn nnnnnannng
      661 gcnccnnnnc nnannnnnnn nnnnnngncn gcnnnaannt gaanngnntn annnnannnn
      721 nnnnnnnnna annnnnatnn ncgnnnnnnn nnnnnngnnn nannnnnnnn nnnnnnnnnn
      781 atggnnannn nncagnnnnn nnnnnnnnnt gtnnanccg
//
