LOCUS       sequence-240038-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of CRY2. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            CRY2-TSS...
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        39..114
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             574..583
                     /estimated_length=10
     gap             655..666
                     /estimated_length=12
ORIGIN
        1 gngnnaggac gaaacaccgc ggaaccagtg caccgaaggt tttagagcta gaaatagcaa
       61 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      121 gaattcgcta gctaggtctt gaaaggggtt ggggttgcgc cttttccaag gcagccctgg
      181 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
      241 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
      301 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
      361 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
      421 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
      481 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggnnngtgg
      541 ngngnanngn nnnnntgtnn nnnnnngngn gngnnnnnnn nnngnangnn nnnngnnnta
      601 tnnnnnntnn ntgtntttnn canncccccn cnnnctcntn nnnngnnggn ntnannnnnn
      661 nnnnnngngn nnntaanntg ta
//
