LOCUS       sequence-240046-         398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of PHF21A. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            PHF21A-T...
FEATURES             Location/Qualifiers
     source          1..398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        32..107
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gacgaaanac cgcccggggc cgggtggtga agttttagag ctagaaatag caagttaaaa
       61 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgaattcg
      121 ctagctaggt cttgaaaggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
      181 caggnacgcg gctgctctgg gcgtggttcc gggaaacgca ncggcgccna ccctgggtct
      241 cgcacattct tcacgtccgt tcgnagngtc ancnnnatct tcgccgctac ccttgngnnn
      301 cccccggcga cncttccngn nnngccccta anncngnaan nttcnnngnn nnnnnngnnn
      361 ngncgnacgn gncnnnnnnn ngccgcacgn nnnannag
//
