LOCUS       sequence-240064-         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            VEGFA-TS...
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        78..153
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 actnnaaann atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgtc
       61 ggaggtcgtg gcgctgggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      121 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattcgctag ctaggtcttg
      181 aaaggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
      241 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
      301 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
      361 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
      421 aacggaagcc gcacgtctca ctagnaccct cgcagacgga cnnngncagn nannaatggn
      481 ngcgcgccga ccgcgatggn ctgtggccaa tancggctgc tcagcagggg cgcgccnaga
      541 gcagcggccg ggnaaggggc ggngngnnan nngnngtgng
//
