LOCUS       sequence-240076-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of ZBED3. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            ZBED3-TS...
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        63..138
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        169..679
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     598..616
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             728..913
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 tnnnnttttg nntttttata tcttgtggaa ggacgaaaca ccgtcagcga ggcgcctgga
       61 gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      121 gtggcaccga gtcggtgctt ttttgaattc gctagctagg tcttgaaagg ggttggggtt
      181 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
      241 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
      301 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
      361 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
      421 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
      481 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
      541 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
      601 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
      661 caccgacctc tctccccagg accggtgatc cgccaccatg gatgctaagt cactaactgc
      721 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg
      781 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa
      841 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa
      901 gggagaagag cccggcggtt ccggcggagg gtcgatggac aagaagtaca gcatcggcct
      961 ggccatcggc accaactctg tcggctgggc cgngatcacc gacgagtaca aggggcccag
     1021 caagaaattc aaggnncggg caaccccgaa cgngnnanca nccagaanan nntga
//
