LOCUS       sequence-240084-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs58692659 (ZFAND3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs586926...
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        64..139
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        170..680
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atnnntttnn tgnnttttat atnttgtgga aggacgaaac accgatatgg gcaggcagtt
       61 gccgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      121 agtggcaccg agtcggtgct tttttgaatt cgctagctag gtcttgaaag gggttggggt
      181 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
      241 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
      301 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
      361 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
      421 gtctcactag taccctcgca aacggacagc gccagggagc aatggcagcg cgccgaccgc
      481 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc caagagcagc ggccgggaag
      541 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggg
      601 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
      661 tcaccgacct ctctccccag gaccggtgat ccgccaccat ggatgctaag tcactaactg
      721 cctggtcccg gacactggtg accttcaagg atgtatttgt ggacttcacc agggaggagn
      781 ggaagctgct ggacactgct cagcagatcg tgtacagaaa tgtgatgctg gagaactata
      841 agaccctggt ttccttgggt tatcagctta ctaagccaga tgtgatcctc cgggtggaaa
      901 aagggaaaaa agcccggggg ggttccgggc ggaggnnnaa agggaaa
//
