LOCUS       sequence-240090-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-g...
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        125..200
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             652..668
                     /estimated_length=17
     gap             670..680
                     /estimated_length=11
     gap             689..719
                     /estimated_length=31
     gap             759..772
                     /estimated_length=14
ORIGIN
        1 gnngtttnnn nntatntttt aaaatggact atcatatgct taccgtaact tgaaagtatt
       61 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgttcca ggccggtcag
      121 ctgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      181 aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa ggggttgggg
      241 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
      301 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      361 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      421 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      481 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      541 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      601 ggggcggtgc gggaggcggg gtgtggnncg nnanngngnn nccngnncca gnnnnnnnnn
      661 nnnnnnnntn nnnnnnnnnn cnngaancnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      721 tcnnancnct ctcnnnannn nnnngnnccn ncnnnnngnn nnnnnnnnnn nnannng
//
