LOCUS       sequence-240092-        1651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-g...
FEATURES             Location/Qualifiers
     source          1..1651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        127..202
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        233..743
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     662..680
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      756..765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             792..977
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 ttgnagtttt aaattatntt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
       61 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgccc gggccccgct
      121 ctgagggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      181 aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga aaggggttgg
      241 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
      301 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
      361 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
      421 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
      481 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
      541 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
      601 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
      661 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
      721 gaatcaccga cctctctccc caggaccggt gatccgccac catggatgct aagtcactaa
      781 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg
      841 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact
      901 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg
      961 agaagggaga agagcccggc ggttccggcg gagggtcgat ggacaagaag tacagcatcg
     1021 gcctggccat cggcaccaac tctgtcggct gggccgtgat caccgacgag tacaaggtgc
     1081 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     1141 tcggcgccct gctgttcgac agcggaaaaa cagcccaagg ccacccggct gaagagaacc
     1201 gccagaaaaa aatccccgaa cggaagaacc ggatctgcnn tnggcaagag atcttcccca
     1261 acgaangggc caaggggaag aagcttcttc ccaagaatgg aaaaatcctt cctgggggaa
     1321 annnaataaa aaccaaaagg ggcccccatt tttcgggaaa tcttgggaca aggtgggcta
     1381 cccaaaaaaa ccccccacct cncccctgaa aaaaaaacgg ggggaaaccc ccaaaaggcc
     1441 caattgggtt taattttggc cccngcccca atgaaaaatt ccggggccct ttctgtaaaa
     1501 ggggcntaaa accccnaaac aattggnaaa agttttctcc ctttgnggga caatacccca
     1561 ttttttggaa aaccttaacc cttgccgggn gtgaccccaa atccttttcc ccggaaaaaa
     1621 aacaaaggcc taaaaacctc caccccctgg g
//
