LOCUS       sequence-240098-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs11257655-guide1
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        80..98
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        150..168
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        255..466
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             483..530
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttnnttnttg nnttttatat cttgtggang gacgaaacac cggtggaaag gtagctgtgg
       61 gagttttaga gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa
      121 ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa
      181 gtggcaccga gtcggtgctt tttttggatc ctaggtcttg aaaggagtgg gaattggctc
      241 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      301 ggtcggcaat tgatccggtg cctaaagaag gtggcgcggg gtaaactggg aaagtgatgt
      361 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      421 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggcgt acggccacca
      481 tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gacaagaagt
      541 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
      601 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      661 aaaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      721 aaagaaccgc caaaaaaaga tacaccagac ggaaaaaccg gatctgctat ctgcaagaga
      781 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg aaagagtcct
      841 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtgaacg
      901 aggtggccta ccacganaag taccccacca tctaccaccg gaaaaagaaa cggggggaca
      961 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccaaatg atcaagttcc
     1021 ggggcccctt cctgatcgag ggggaaccgg aacccnggan a
//
