LOCUS       sequence-240102-        1425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs4237150 (GLIS3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs4237150-guide2
FEATURES             Location/Qualifiers
     source          1..1425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        137..155
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        207..225
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        312..523
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             540..587
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1106..1117
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttanattat nttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
       61 ttcttggctt tatatatctt gtggaaagga cgaaacaccg atgagttatg actctagatg
      121 ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata
      181 aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg
      241 gcaccgagtc ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg
      301 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      361 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      421 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      481 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga
      541 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca
      601 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      661 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      721 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      781 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      841 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      901 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtgggacgag
      961 gtgggcctac cacgaagaag taccccacca tctaccacct gagaaaagaa actgggtgga
     1021 cagcaccgac aaggcccgac ctgccggctg atctatctgg nccctggccc cacatgaatc
     1081 aagtttccgg gggccacttn cctgaatcga agggcgaacc tggaaccccn ancaacaggc
     1141 gacnngggaa caagcctgtt tcatcccngc tgggtgcaag acctancaac ccagcctggt
     1201 tccaaggaaa aacccccatn naaccgccag ccgggggtgg aaacccccaa gggccattcc
     1261 ctggtctttc ccaaaattga aacaaaaaaa caaaaacggc tttggaaaaa atcttgaatc
     1321 ggccccaact tgcccccggc naagaaaaaa aaanaaatgg gccctggttt tcgggaaacc
     1381 nggtattggc cccttgagac cctggggggc ctgggaancc caaat
//
