LOCUS       sequence-240116-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7163757 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A/B-enh2-guide1
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..162
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        214..232
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        319..530
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             547..594
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnntttta aattatnttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
       61 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgcttc tctactgacg
      121 acatccgttt tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt
      181 taaaataagg ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg
      241 ccaagtggca ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg
      301 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      361 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      421 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      481 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc
      541 accatgaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggacaag
      601 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      661 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      721 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      781 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      841 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      901 tccttcctgg tggaagagga taagaagcac gagcgncacc ccatcttcag gcacatcgtg
      961 gaacgaggtg gcctaccacn nataagtacc ccaaccatct aacacctgga taagaaagct
     1021 gnnggnncag caaccgacaa gggccgacnt gcggcnngat nnatnggggc cttgtgccag
     1081 agtgatcaag ttccgggggc cacttcctgg atcgangggc gaccatgaac cccgaacann
     1141 gccgactttg aacgacnntg ttcatcccgc ttgggggagg acctagaaac tcctggattc
     1201 aaggaaagaa ccccgtcaac gccccggtgg gtggaaacgc gaagaaccat ccgggcttag
     1261 ccaaanctaa ngnaaangcg aaacggggtg gaaaaaccnn nttcccccat gttnnnncna
//
