LOCUS       sequence-240122-        1402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7163757 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A/B-enh2-guide4
FEATURES             Location/Qualifiers
     source          1..1402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        139..157
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        209..227
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        314..525
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             542..589
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tttaaaatta tnttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
       61 tttcttggct ttatatatct tgtggaaagg acgaaacacc gcggagacaa gtctgtgtca
      121 ggttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa
      181 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag
      241 tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc
      301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      481 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggcgta cggccaccat
      541 gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg acaagaagta
      601 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
      661 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcgtnaa
      721 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
      781 gagaaccgcc agaagaagat acaccgacgg aagaaccgga tctgctatct gcaagagatc
      841 ttcagcaacg agatggccag gtggacaaca gcttcttcca cagactggga agagtcccgt
      901 nntggtggaa gcngnannca aagccacgag cggcacccca tcttcntgca acatccnngg
      961 aacgaggtgn acctaccacg agaannaacc ccaccatcta ccagctgann tannaaannn
     1021 natggaacgn ncangacaga ggccgaactg gcggcatgat ccatcntggc cttggcacca
     1081 catggatcaa gttccgggag gctcntttnt gantnanngg gcaacnngna acnncgaaan
     1141 ntaggnaacg ggggaacaag ctagttcnnn ccggcctggt gccgaacctn nngaacnnct
     1201 tgttccgann aaaaacccnn ntccaagcct caaacggaat tagggccccn ttatccatcc
     1261 ggttcggnnc cggaactgaa ggnaaaggca taaggctggg aaaaaccgtt aagtccgccn
     1321 ttttcccggg ttaaggaaaa aaaaacagta ngncttagac nangaactgg tataaccctt
     1381 taaaattang aanannnnac ac
//
