LOCUS       sequence-240124-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs17205526 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A/B-enh3-guide1
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        118..136
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        188..206
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        293..504
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gnactannnt anncttaccg taacttgaaa gnatttcgat ttcttggctt tatatatctt
       61 gtggaaagga cgaaacaccg aagaagcata ttcagctgac gttttagagc taggccaaca
      121 tgaggatcac ccatgtctgc agggcctagc aagttaaaat aaggctagtc cgttatcaac
      181 ttggccaaca tgaggatcac ccatgtctgc agggccaagt ggcaccgagt cggtgctttt
      241 tttggatcct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
      301 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
      361 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      421 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      481 aacgggtttg ccgccnnaac acaggcgtac ggccaccatg aaaaggccgg cggccacgaa
      541 aaaggccggc cagncanaaa agaaaaagga caagaagtac agcatcnggc ctggncatcn
      601 nnnncaactc tgtgnnctgn gccgtgatca ncgacganna nnangngccn annangaann
      661 tcnnngngnt gnnnnnnncn aaccggnnnc agcatcnnnn anancctgat cggancncct
      721 gctgttcga
//
