LOCUS       sequence-240128-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs17205526 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A/B-enh3-guide3
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        114..132
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        184..202
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        289..500
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     gap             677..687
                     /estimated_length=11
     gap             730..739
                     /estimated_length=10
ORIGIN
        1 tannatnnnn ttaccgtaac ttgaaagnat ttcgatttct tggctttata tatcttgtgg
       61 aaaggacgaa acaccgtgtt agcatcaagg cctcaagttt tagagctagg ccaacatgag
      121 gatcacccat gtctgcaggg cctagcaagt taaaataagg ctagtccgtt atcaacttgg
      181 ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt gctttttttg
      241 gatcctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
      301 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
      361 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      421 agggtggggg agaaccgtat ataagtgcag tantcgccgt gaacgttctt tttcgcaacg
      481 ggtttgccgc cagaacacag gcgtacggcc accntgaaaa ggccggcggc cacgaanaan
      541 gncggccagn caaaaaagaa aaaggacaag angtacngna tcggcctggc catcggcncc
      601 aactctgtgg gctggnncgn gatcaccgac nagnnnnnnn gnnnnnnnng annnnnangg
      661 ngctgnnnnn nnncnannnn nnnnnnntcg anaananccn nnnnnnnagn nntgctgnnt
      721 cgacngnngn nnnnnnnnna ggncnnnncg tnnnnaanan aancnnncnn annaanat
//
