LOCUS       sequence-240130-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 VPS13C-TSS-guide1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        56..74
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        126..144
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        231..442
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             459..506
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tngaaaggac gaaacaccgg cggcgcctac aaagcctcgt tttagagcta ggccaacatg
       61 aggatcaccc atgtctgcag ggcctagcaa gttaaaataa ggctagtccg ttatcaactt
      121 ggccaacatg aggatcaccc atgtctgcag ggccaagtgg caccgagtcg gtgctttttt
      181 tggatcctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
      241 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
      301 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
      361 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
      421 cgggtttgcc gccagaacac aggcgtacgg ccaccatgaa aaggccggcg gccacgaaaa
      481 aggccggcca ggcaaaaaag aaaaaggaca agaagtacag catcggcctg gccatcggca
      541 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
      601 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
      661 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
      721 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
      781 tggacgacag cttcttccac agactggana gtccttcctg gtggaagagg ataataagca
      841 cgagcggcac tccatcttcg gcaacgtcgt ggacganntg gcctaccacg agaagtacct
      901 caccatctac cacctgagaa aaanctggtg gactgcaccg acaaggccga cctgcggctg
      961 atctatctgg ccctggccca aatgatcaag ttccggggcc acttcctgat caagggcgac
     1021 ctgaactccg acataggnna cggggacaag ctgttcttcc agcttgtgca gaactacnac
     1081 cagctggttn aagaaaactc nnncaacntc aagcncatgg aagccnaaga ccnnct
//
