LOCUS       sequence-240134-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 VPS13C-TSS-guide3
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        74..92
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        144..162
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        249..460
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     gap             857..868
                     /estimated_length=12
     gap             896..909
                     /estimated_length=14
ORIGIN
        1 cnnnnnnnnn tatancttgn ggangganga aacnccggcc ctctagaggc cgacgggttt
       61 tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg
      121 ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca
      181 ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg gctccggtgc
      241 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
      301 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
      361 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
      421 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc accatgaana
      481 ggccggcggc cacgaaaaag gccggccagg caaannnnan nnangannan aantacagcn
      541 tcgnnctggc catcgnnacc aactctgtgg nctgggccnt gatcaccgac nantncnang
      601 tgcccncnca gaaattcnng gtgctgngnn ncaccnanng gnncngcnnn nngaanannn
      661 tnnnngnann cntgcngnnc nanncnnnna nnnnnccnan gccnnncngn tgananaann
      721 nnnnnannnn natacncnnn acgcnnnacn nnnanctgnn ntctgnnnga gatnntcnnn
      781 ncgagatggn nnnggagnan nannncntcn tncnnnnnan tggnnnnnnc nnnnnngnnn
      841 nanannnnnn gnanncnnnn nnnnnnnncc nnncttngnn nnnnntnngn nannannnnn
      901 nnnnnnnnna anancnnnnc cnnnnnannn nna
//
