LOCUS       sequence-240136-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 VPS13C-TSS-guide4
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        78..96
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        148..166
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gap             501..512
                     /estimated_length=12
     gap             650..674
                     /estimated_length=25
     gap             690..704
                     /estimated_length=15
ORIGIN
        1 ttnnnngngn nntatatatc ttgnggangg angaaacacc gtcctccgtc ggcctctaga
       61 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      121 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      181 ggcaccgagt cggtgctttt tttggatcct aggtcttgaa aggagtggga attggctccg
      241 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      301 tcggcaattg atccggtgcc tanagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      361 tgtactggct ccgccttttt cccgagggtg ggggagaacc gnatataagt gcnntanncn
      421 ccgngaacgt tctttttcnn ancgnnnnng ccnncnnanc ncangnntac nnnnnccatg
      481 acanngccgg ngnccnnnaa nnnnnnnnnn nncnnaacnn annnnnacnn gacnnnncnn
      541 atcgccnngg nnncnnnnnn nntctgngnn cngnnnnnng ancnnnnnnn ncnnnnnnng
      601 cccnncanga atnnnnngng ncnnnnnnnn cncnngnnnn ncnnnnnnan nnnnnnnnnn
      661 nnnnnnnnnn nnnnccnncn nnnnnnnccn nnnnnnnnnn nnnncnnann ncnnnncnnn
      721 nnnananccn nnnncnnnnn nnngcannnn ncngnnnnnn annttctnnn nnnnncnnnn
      781 ncnnnngaan ncnncttnnn nnncnnnnng nnngnnnncn ntnngnnnnc
//
