LOCUS       sequence-240138-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A-TSS-guide1
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        56..74
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        126..144
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        231..442
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             459..506
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tgnaaaggac gaaacaccgt ccctgtccct ctgccacggt tttagagcta ggccaacatg
       61 aggatcaccc atgtctgcag ggcctagcaa gttaaaataa ggctagtccg ttatcaactt
      121 ggccaacatg aggatcaccc atgtctgcag ggccaagtgg caccgagtcg gtgctttttt
      181 tggatcctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
      241 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
      301 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
      361 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
      421 cgggtttgcc gccagaacac aggcgtacgg ccaccatgaa aaggccggcg gccacgaaaa
      481 aggccggcca ggcaaaaaag aaaaaggaca agaagtacag catcggcctg gccatcggca
      541 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
      601 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
      661 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
      721 ccagacggaa gaaccggatc tgctatctnn gagagatctt cagcaacgag atggccaagg
      781 tggacgacag cttcttccac agactggagc agtccttcct ggtggaatag ganaganctc
      841 gacagnnngc catcttcagc annatcgtgg aanaggtggc ctaccacnnn naatgagcgc
      901 accatcnncn accttagtaa taanactgnn ggacnccact nacaatgnnn gancatagtg
      961 cagannnatc tggcgctggg tccacatgaa tcangtnncn nngcnatttc cggatcgaag
     1021 ccgcacccca aacccannnn ntcacctccn ncannnnatc cgtctnnnc
//
