LOCUS       sequence-240140-        1375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A-TSS-guide2
FEATURES             Location/Qualifiers
     source          1..1375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        80..98
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        150..168
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        255..466
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             483..530
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1042..1053
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tnnnttnntg gntttatata tcttgtggaa ggannaaaca ccgcaggcag cgcccctctg
       61 ctgttttaga gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa
      121 ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa
      181 gtggcaccga gtcggtgctt tttttggatc ctaggtcttg aaaggagtgg gaattggctc
      241 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      301 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
      361 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      421 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggcgt acggccacca
      481 tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gacaagaagt
      541 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacaagt
      601 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      661 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      721 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
      781 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
      841 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggaac
      901 gaggtggcct accacgagaa gtaccccacc atctaccacc tgaaaaaaaa aactggnggg
      961 acagcaccga caagggccga cctggcggct gatctatctg gccctggccc acatgatcaa
     1021 gttccggggg ccacttcctg gatcgagggc cgacctgaac cccnaacaac ggcgacgnng
     1081 gacaagctgt tcatccaggc tggnggaaaa cctacaacca ggctggttcg aggaaaaacc
     1141 ccnatcaacg ccaggggnng gggaacgcca aggccnttcc tggtctggcc aaactgaacc
     1201 aaaaacaaaa cggttggaaa aatctgaatc cccccacctg ccccggnaan naaaaaaaaa
     1261 agggcccggt ttcggcaacc tggattggcc ctgnaacccg ggggcctnga acccccaaat
     1321 ttcaaanaaa aantttncaa acctgggcca aagaaggccc aaaatgggcc nntga
//
