LOCUS       sequence-240144-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A-TSS-guide4
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        52..70
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        122..140
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        227..438
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             455..502
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aangacgaaa caccgcactg gcaagcgctc ccaggtttta gagctaggcc aacatgagga
       61 tcacccatgt ctgcagggcc tagcaagtta aaataaggct agtccgttat caacttggcc
      121 aacatgagga tcacccatgt ctgcagggcc aagtggcacc gagtcggtgc tttttttgga
      181 tcctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
      241 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
      301 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      361 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      421 tttgccgcca gaacacaggc gtacggccac catgaaaagg ccggcggcca cgaaaaaggc
      481 cggccaggca aaaaagaaaa aggacaagaa gtacagcatc ggcctggcca tcggcaccaa
      541 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
      601 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
      661 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
      721 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
      781 cgacggcttc ttccacagac tggaaagagt ccttcctggt ggaagaggaa atgaagcacg
      841 agcgtganct ccatcttccg gnacatcatg naacgagnnt ncacaaccac gagaaagtac
      901 tccangatcc aacaactgag annaaact
//
