LOCUS       sequence-240146-        1582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4B-TSS-guide1
FEATURES             Location/Qualifiers
     source          1..1582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        75..93
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        145..163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        250..461
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             478..525
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnttnnnn ntttttnttg tggaaggacg aaacaccgcc agtgcatgca gacctccgtt
       61 ttagagctag gccaacatga ggatcaccca tgtctgcagg gcctagcaag ttaaaataag
      121 gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg gccaagtggc
      181 accgagtcgg tgcttttttt ggatcctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggcgtacggc caccatgaaa
      481 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaaggacaa gaagtacagc
      541 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      601 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      661 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      721 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      781 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      841 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      901 ggcctaccac naaaagtacc ccaccancta caacctgaga aagaaactgg tggacagcac
      961 cgacaaggcc gacctgccgc ntgatctatc nngccctgga cccacatgat caagttccgg
     1021 ggcccacttc ntggatcaag ggcgaacttg aaccccaaca acagcgacgt gaacaagctg
     1081 ttcatcannt tgntgcaaga ctacaaccan ntggttcgag gaaaacccat tcaacgccag
     1141 gtaggcgggg aaccnaagag cnntcctgtc cnaccaaant gaagcaagan nnaaacgctg
     1201 gtgaaaatnn nntncgccac cattccccgg ccggaaaaaa aanggaccgt tgtccggaac
     1261 tcggattgac ctgaancctt ggccgtagnn cccnncttna aangagaact tacngaccgc
     1321 cggaaaaaag accaagatgg tttagaaaaa anngaaannc caaataaaaa aaccggtaaa
     1381 ggatttgggg gccgaaaagg tanaagcaaa gcccaaaccg gtaattggcc tacggaanaa
     1441 ntggnaaacc ccttcccntc aaagataanc tttaggaann tttaacgata aaagcctggg
     1501 cttccaccgg nactttcggt tttccgannn taaaaataag gcaaatgnnc cantgggata
     1561 aacgggnnat nnttgaattt na
//
