LOCUS       sequence-240148-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4B-TSS-guide4
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..19
                     /estimated_length=18
     misc_RNA        67..85
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        137..155
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        242..453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             470..517
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnnnnnnn nnnnnnnnna cgaaacaccg gcagacctcc cggcgtcgtg ttttagagct
       61 aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata aggctagtcc
      121 gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg gcaccgagtc
      181 ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
      241 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      301 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      361 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      421 ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga aaaggccggc
      481 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca gcatcggcct
      541 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
      601 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
      661 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga caaccgccag
      721 aagaagatac accagacaga agaaccggat ctgctatgag cnnnagatct tcagcaacta
      781 aatggccnng ggggacgaca gcttcttcnn nagactggaa gactccttcc tggtgcnana
      841 ggatacaang cacaannnnc accnnatctt cgacaacatn nagggacann ntggactant
      901 ncgaataatn nccctnncat tctannnnnn gagtaataan cntnctgt
//
