LOCUS       sequence-240152-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs1401419 (CRY2 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs1401419-guide2
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        126..144
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        196..214
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        301..512
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             529..576
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttanatnn actatnatat gcttaccgta acttgaaagt atttcgattt cttggcttta
       61 tatatcttgt ggaaaggacg aaacaccgaa actaggccac aagtctgcgt tttagagcta
      121 ggccaacatg aggatcaccc atgtctgcag ggcctagcaa gttaaaataa ggctagtccg
      181 ttatcaactt ggccaacatg aggatcaccc atgtctgcag ggccaagtgg caccgagtcg
      241 gtgctttttt tggatcctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
      301 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat
      361 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      421 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      481 tttttcgcaa cgggtttgcc gccagaacac aggcgtacgg ccaccatgaa aaggccggcg
      541 gccacgaaaa aggccggcca ggcaaaaaag aaaaaggaca agaagtacag catcggcctg
      601 nncatcggca ccaactctgt gggctgggcc gtgatcaccg acnagtacaa ggtgcccagc
      661 aagaaattca aggtgctggg caacaccgna gcggcacagn atcaagaaga acctnatcgg
      721 agccctgctn tncnacagng gcgaaacagc cgaggccacc cggctgaaga gaaccgccgg
      781 aagaagatac accnnaacgg aagaaccagg aacnngctat ctgncannag atcttcagca
      841 acaaacatgn cnaagngtgg anntacagct tccttccaca gaactgatat aattcntttc
      901 cngganntaa caatgnntnn gaannncnna nnnnnaaccc nannntttag taaaannctt
      961 gggnnataga nngnnnttac ctacnnnnna ttaccctt
//
