LOCUS       sequence-240154-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs1401419 (CRY2 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs1401419-guide3
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        136..154
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        206..224
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        311..522
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             539..586
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 annttatngt tttannannn actatcatat gcttaccgta acttgaaagt atttcgattt
       61 cttggcttta tatatcttgt ggaaaggacg aaacaccgct ctgtttgcta ttggcctcgt
      121 tttagagcta ggccaacatg aggatcaccc atgtctgcag ggcctagcaa gttaaaataa
      181 ggctagtccg ttatcaactt ggccaacatg aggatcaccc atgtctgcag ggccaagtgg
      241 caccgagtcg gtgctttttt tggatcctag gtcttgaaag gagtgggaat tggctccggt
      301 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      361 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      421 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      481 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggcgtacgg ccaccatgaa
      541 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggaca agaagtacag
      601 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      661 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      721 cctgatcgga gccctgctgt tcgacagcgg cgaacagccg aggccacccg gctgaagaga
      781 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      841 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      901 gtggaaagag gatagaaagc antanngnna cgccatctnn ngcaacat
//
