LOCUS       sequence-240156-        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs1401419 (CRY2 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs1401419-guide4
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        141..159
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        211..229
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        316..527
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             544..591
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 anttttaaat tatnttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
       61 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgacaactc tgccaagaaa
      121 actgttttag agctaggcca acatgaggat cacccatgtc tgcagggcct agcaagttaa
      181 aataaggcta gtccgttatc aacttggcca acatgaggat cacccatgtc tgcagggcca
      241 agtggcaccg agtcggtgct ttttttggat cctaggtctt gaaaggagtg ggaattggct
      301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      361 gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggcg tacggccacc
      541 atgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa ggacaagaag
      601 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      661 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      721 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      781 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      841 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      901 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggga
      961 cgaggtgggc ctaccacnag aagtaccnca ccatcctacc acctggagaa anaaactggg
     1021 tgganngcac cgacaaggcc gaactggngc ttgatctatc tgggccctgg gcccanaagg
     1081 atcaagttcc gggggccact ttcctggatc caggggcgaa ctggaacccc ggacaactcg
     1141 taaatgggaa aaagctgttt caccaanttg gttgcanacc tacaacccac tggttttcaa
     1201 gggaaaaacc ccatttaaac ncancggtgg gtggaacccc aangcc
//
