LOCUS       sequence-240162-        1425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 PHF21A-TSS-guide1
FEATURES             Location/Qualifiers
     source          1..1425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        77..95
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        147..165
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        252..463
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             480..527
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attnnnggnt ttntatatct tgtggaagga cgaaacaccg agccgggcga gagggactgg
       61 ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata
      121 aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg
      181 gcaccgagtc ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg
      241 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      301 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      361 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      421 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga
      481 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca
      541 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      601 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      661 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      721 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      781 tcagcaacga gatggccaan gtggacgaca gcttcttcca cagactggaa nngtccttcc
      841 tggtggaaga ggataagaag cacgaacggc accccatctt cggcaacatc gtggacgagg
      901 tggcctacca cgagaagtac cccaccatct accacctgaa gaaagaaact ggtggacagc
      961 accgacaagg cngacctgcg gctgatctat ctgggccctg gcccnnataa tcaagttccg
     1021 ggggccactt tnnnnncgaa ggcnacctgg aacctcgaca acagcgaact gggaacaacc
     1081 ggttcatcca gntntggcag acctacaacc cgnnggttcg aagaagaacc nntaancccg
     1141 gnngccgggn aanccaaggc cattccggnc tagcnnaatg gaccaagaat gaaagggnng
     1201 ggaaantctg aatccccacc tttcccggna anaaaaagaa aaggcgcggt ccgaaacccg
     1261 gttgcccgga accaggggcc tagaccngca attncaaaga ngttctgaac ttggccaaga
     1321 gttggaacgg ggnnnaagga aggggaccct caaaaaaaaa aaaggnaaaa aacgtttggt
     1381 cgaagttngt taggtaaaaa cccagtttta tttagcccgn gaaaa
//
