LOCUS       sequence-240164-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 PHF21A-TSS-guide2
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        49..67
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        119..137
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gap             605..615
                     /estimated_length=11
     gap             633..642
                     /estimated_length=10
     gap             760..769
                     /estimated_length=10
     gap             851..875
                     /estimated_length=25
     gap             877..887
                     /estimated_length=11
ORIGIN
        1 gnngaaacac cgcctgggcg gagggatccc cgttttagag ctaggccaac atgaggatca
       61 cccatgtctg cagggcctag caagttaaaa taaggctagt ccgttatcaa cttggccaac
      121 atgaggatca cccatgtctg cagggccaag tggcaccgag tcggtgcttt ttttggatcc
      181 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
      241 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg
      301 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      361 gggggagaac cgtatataag tgcagtantc nccgngancg ttctttttcg caacgggttt
      421 gccgccanaa cncaggcgta cggncaccnt gannnggccg gcggccacna aanangncgg
      481 ncagccaaan nccaannann annanaanta cnnnnnccnn ctgnncnncg gcaccaantc
      541 tgngnnntgn nncgngntnn ccnacgannn cnnnnngncc nncncnanan tnnnnnngnn
      601 gnncnnnnnn nnnnngnnnn ncnatnnnnn nannnnnnnn nnancgctgg ntgnncnnnn
      661 nngnccaann nccnnnncnn anccgnntga nnnnnnnggn cacnnnnaga nanacnnnag
      721 nnnnnncccn gntnnngcnn cnnntgnagn tnnnnangcn nnnnnnnnng nctgngnngn
      781 anannnnntn nntnnnnnnn nngnngcacc ncnncctgng nnannnnnnn nncannnnnn
      841 nccnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnngnnnn nnnnnnntgg nnnaannnnc
      901 nnnnnngant nnctannntn ntnnnna
//
