LOCUS       sequence-240166-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 PHF21A-TSS-guide3
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        76..94
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        146..164
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        251..462
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     gap             785..794
                     /estimated_length=10
     gap             871..882
                     /estimated_length=12
     gap             956..973
                     /estimated_length=18
ORIGIN
        1 ttgnncnntt nnntnnnnnn gtgnnnngan gaaacaccga aagcggggac gagggcgagt
       61 tttagagcta ggccaacatg aggatcaccc atgtctgcag ggcctagcaa gttaaaataa
      121 ggctagtccg ttatcaactt ggccaacatg aggatcaccc atgtctgcag ggccaagtgg
      181 caccgagtcg gtgctttttt tggatcctag gtcttgaaag gagtgggaat tggctccggt
      241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      301 ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      421 gtgaacgttc tttttcgcaa cgggtttgcc gccngaacac aggcgtacgg ccaccatgaa
      481 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag annnnngann anaagtacag
      541 catcggcctg gncatcggnn cnnnctctgt gggctgggcc gtgatcnccg acnantannn
      601 ngcgccnnnn nagannttnn nggtgctggn cancnncnac ngnnnnagnn tcnnnnnnaa
      661 cntgatnnga nccntgnngn ncnaccgcnn nnaancngnc nannccacnn ggntgannan
      721 aacccnnngn nnnnnatann ncncacgnnn nnatccnnnn tnnnnnnnnc tgcnnnannt
      781 nntcnnnnnn nnnnatggnn nnngcnnnan ganagnttnn ttnnngnnac tgnnnnaann
      841 cntncnctgg nngnannang nnnnnnannc nnnnnnnnnn nncnncntnt ncnnnnnann
      901 gccnnnnnnn angnnnnccc acnnnnntna annanccnnc cnnnnnnnan nntgannnnn
      961 nnnnnnnnnn nnntngnnac ncnnnnnnnc canntnnnnn nnntnctntn tgtnnnttgn
     1021 nnnnnnnanc cnnnntnnnt nntcnnntnn nnnaannna
//
