LOCUS       sequence-240168-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of PHF21A. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 PHF21A-TSS-guide4
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        64..82
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        134..152
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gap             804..815
                     /estimated_length=12
     gap             848..863
                     /estimated_length=16
     gap             916..925
                     /estimated_length=10
ORIGIN
        1 tntcttgnnn gnnnggnnga nncnccggct gggaaagcgg ggacgagttt tagagctagg
       61 ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg ctagtccgtt
      121 atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca ccgagtcggt
      181 gctttttttg gatcctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
      241 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
      301 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      361 ctttttcccg agggtggggg agaaccgtat atnantgcan tantcnccgt gaacgttctt
      421 tttcgcaacg ggtttgccgc canaacncan gcgtacggnc accatganna ggccggnggc
      481 cacgaaanag gccggncagn cnaannanan nnnnnannnn aantacagcn tcnnnctggn
      541 catcgnnncc aactctgngn nctgngccnn gnncaccgnc nantactacg tgcccacctc
      601 gancnnnnng gtgctnnnnn ngnnnnancn gnnnnccntc nnnnnnaanc nnatnngnnn
      661 ctngnnnnnc nacnnnnnnn nannnnngcn acnncnnnnn nctgaanana acnnnnnnnn
      721 nannanncnc nnnnnnannc ncnnnannng ctnnnnngnn tnannnnttc ncnnnnnana
      781 ngnnnnctnn nnnannannn nntnnnnnnn nnnnncnnna ntanccctnn cntgnnggaa
      841 nannatannn nnnnnnnnnn nnnccctntn cnnnnngnnc ctncgnnnnn nnnnctnnnn
      901 nnnnaaannn ncnncnnnnn nnnnntnncn nnnnnngnnn nnnnntncnn anaa
//
