LOCUS       sequence-240170-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs12189774 (VEGFA GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs12189774-guide1
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..162
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        214..232
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        319..530
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             547..594
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttnagtttta aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
       61 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtaag tcagcactaa
      121 tggggggttt tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt
      181 taaaataagg ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg
      241 ccaagtggca ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg
      301 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      361 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      421 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      481 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc
      541 accatgaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggacaag
      601 aagtacagca tcggcctggc catcgggacc aactctgtgg gctgggccgt gatcaccgac
      661 gagtacaagg tgcccagcaa gaaagtcaag gtgctgggca acaccgatcg gcacagcatc
      721 aagaagaacc tgatcggagc cctgctnttc gacagcggcc gaaacagccg aggccacccc
      781 ggctgaaaga gaacccccca gaannatgag acatcnncac ggacataacc gggatctggc
      841 tatcttgnnn nganntcctt cagcaatcaa gtacggatca agggcggnng aacagccttc
      901 tttccgctaa actggnanng agtacctttc tcggtgggaa aaaggatcag caaaccacga
      961 gaccgtgtaa ccccatnntt cctgaaccat cnnttgnnnn aa
//
