LOCUS       sequence-240172-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs12189774 (VEGFA GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs12189774-guide2
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        140..158
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        210..228
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 gttntnnntt atnttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgactaatgg gggtggtacc
      121 tggttttaga gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa
      181 ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa
      241 gtggcaccga gtcggtgctt tttttggatc ctaggtcttg aaaggagtgg gaattggctc
      301 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      361 ggtcggcaat tgatccggtg cctagagaan gtggcgcggg gtaaactggg aaagtgatgt
      421 cgtgtactgg ctccnccttt ttcccgaggg tgggggagaa ccgtatatan nngnannant
      481 cnccnnnnan nntctttttc ncnncnggtt tgncgncnna acacaggcnt acngccnccn
      541 tgaaaangcc ngnnnnnnnn annaangncn nnnnnncnnn naanannnnn gannnnannt
      601 ncngcntcnn nctgcncatc ggcaccanct ctgtgggctg nnccgtgatc accgacnagn
      661 acaaggngnc cannnnnnnn ttnnnngngc tggnnnacnn cnancggnnc nnnatcnnna
      721 nnannnnnan nnnanncctg ctgntcgacn gnnnnnaaac ntncnangnc nnccggctga
      781 nnananncnn cngannaana tnnncanann gnannannnn tnatctgntn tntgnnanta
      841 natcttnann nnnnananng nnnnggtgnn annacnnctt cttccnnnnn nngnananan
      901 nnnttnntng tnnaanagnn anannaacca ctannnnnnn ncncntnctn nnnnnnnttn
      961 nnnnga
//
