LOCUS       sequence-240174-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs12189774 (VEGFA GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs12189774-guide3
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..12
                     /estimated_length=11
     primer_bind     43..62
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        159..177
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        229..247
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        334..545
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             562..609
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 annnnnnnnn nnanttgnag ttttaaatta tgttttaaaa tggactatca tatgcttacc
       61 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      121 gctaatgggg gtggtacctg agttttagag ctaggccaac atgaggatca cccatgtctg
      181 cagggcctag caagttaaaa taaggctagt ccgttatcaa cttggccaac atgaggatca
      241 cccatgtctg cagggccaag tggcaccgag tcggtgcttt ttttggatcc taggtcttga
      301 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      361 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
      421 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      481 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      541 cacaggcgta cggccaccat gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa
      601 aagaaaaagg acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
      661 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
      721 gaccggcacg gcatnnngaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
      781 gccgaggcca cccagctgag aagaaccgcc cgaagaagat acaccatacc gtaagaaccg
      841 natctgctat ctgcaagaga tcttcagcan ngagatggcc caagtgggac gacagccttc
      901 ttccacacac tggaatgagt ccttcctggg tggaagagga nnagaagcac taagcggctc
      961 ctcaatcttc cgcaaacgtc gatggatcaa ggtgtcctat ctcnnnnaag gtannngtt
//
