LOCUS       sequence-240176-        2239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs12189774 (VEGFA GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs12189774-guide4
FEATURES             Location/Qualifiers
     source          1..2239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        140..158
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        210..228
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 tnnnnnntta tngttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtctctctc tcattggccc
      121 tcgttttaga gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa
      181 ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa
      241 gtggcaccga gtcggtgctt tttttggatc ctaggtcttg aaaggagtgg gaattggctc
      301 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      361 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaagtggg aaagtgatgt
      421 cgtgtactgg tctccgcctg ttttcgcgac gtggatgggg gcttaaccct gtcattataa
      481 agttgacagt caatccgcac gttgatacag ttctgcctta tttacgccat agcgtgcgta
      541 tgtggcgcag gcccnnncaa accatctacn tggccgttta ccagcgtccc ctacccccca
      601 ctangttaac aatacacgcg gccccccggc gggcttgacg gctcccccaa acccggccaa
      661 caaccaacca aaaaaaggtt ggttcctccc ttggggggtt ccaaacctaa attggttggg
      721 aaccttatta attcaanctt taattaattg ggttaattaa ttaacaaaaa aatttggttg
      781 ggaaaatccc taaattaatt gggggaatta aagggccttt aaaaagcccc aaaggggann
      841 nctaaaggtt tggcctgggg gggggccncc cattttnggg ggggagcccc ncccanatnn
      901 tacccgcggg aagggcnnna aaggcccgcc cngaaaggaa atccctnttt gccccgtttg
      961 gggtttntag ggagggaggg tccccttttt gggnaagggg gggccctccc ctgggcttac
     1021 ggggcaaatt ttcgcccaaa ccccccccgg gggtaaaatc cccgggggaa aagggctttt
     1081 taaaaccccc aaaaaaaaag gcggggtttt ggngggcccc ccccccccca aaagggggcc
     1141 ccaaaagaag gggcaaaaaa aaaaaaattt tnttttcccc caaaaaaaag ggnggggttt
     1201 ttggggcccc ctttttgggg gggggggggc cccaaaaaaa aacccccaaa acccctcccc
     1261 cggggtaaaa cccccncccc ggaggggggc cccaaaaacc cctaaaaggg gccccaaaat
     1321 tttttccnna aaaaaaaggg gaaaaaaaaa gggggaaaaa aaaccccccc ttttgggnga
     1381 aattttaccc gggggggcta aaagggtccc cccccccctt ttggggcccc ctttggggtt
     1441 tttttccccg ggtaaaaccc aaaagggtcc nctggggggg ccccgggaaa aaaaaaaacc
     1501 ccaaagggac ccccccgggg aaaagggggg ggccccccca atcccccccc ccggaggggc
     1561 cccttttggg gaaaaaaagg ggaaagggaa aaaaaccccc ccgggccccc cccaaaaggg
     1621 aaaaaaaggg caaaaaaagg gaaaaattta aacccccaat cccccccaaa aagggaaaac
     1681 cccggtgggg gaaaaaaggg ggaaaaaaan ccccccctgg gggnaaaatt tcccttttcg
     1741 ggccccattt aaattttccc ttttgggccc tcaaaaaggg ggaaaaaggc aaattttccc
     1801 cttatttttc ccaaaaggcc cccaaaaaaa cccnggaaaa aggngaaatt ttgggggggc
     1861 ccccccaaaa aaaattgggg attttggngg gaaaaatccg ggaataaanc caaaggggcc
     1921 cctttttttc ccctttttat cccccccaaa accataaagg aaaaaccntt tgggggggaa
     1981 aaaaaagcaa aagggttttc ccccnttttt ttnccccctt ttgggggagt ttttgggggg
     2041 aaaaaaaaaa aaaacggcgc aaaattttaa anaaaaggaa ttaaagtccc caattctctg
     2101 gaaaaggtcc cggggggggc aaaatccccc cccnccnaat ttcccctttt ttcccccggg
     2161 ggnaaaaaaa acccaatttt cccccgcttt ggggggaaaa acccgggaaa aaaggggttt
     2221 tggggggacc cncttttaa
//
