LOCUS       sequence-240180-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 VEGFA-TSS-guide2
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        125..143
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        195..213
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 ttnnnnangn actatnatat gcttaccgta acttgaaagt atttcgattt cttggcttta
       61 tatatcttgt ggaaaggacg aaacaccggt agcggggagg atcgcgggtt ttagagctag
      121 gccaacatga ggatcaccca tgtctgcagg gcctagcaag ttaaaataag gctagtccgt
      181 tatcaacttg gccaacatga ggatcaccca tgtctgcagg gccaagtggc accgagtcgg
      241 tgcttttttt ggatcctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      301 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
      361 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      421 cctttttccc gagggtgggg gagaaccgta tataagtgcn nnanncnncn ngnannntct
      481 ttttcgcanc gggntngccn ncnnannnnn ngcntacggc caccatgaaa aggccggcnn
      541 ncacnannaa ggnnggnnnn ncaaaaaana nnnnnnncnn nnnnnanagn nncngnctgn
      601 ncatcnnnan nnntctgtgg nntggnncgt gntcncnnna nnagnangng ngtnnannnn
      661 nnntcana
//
